大鼠脑甘氨酸转运体GLYT2基因5'区域的特征分析：一种新型异构体的鉴定。
Characterization of the 5' region of the rat brain glycine transporter GLYT2 gene: identification of a novel isoform.
作者信息
Ponce J, Poyatos I, Aragón C, Giménez C, Zafra F
机构信息
The Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, Spain.
出版信息
Neurosci Lett. 1998 Feb 6;242(1):25-8. doi: 10.1016/s0304-3940(98)00037-8.
We have identified two alternative 5' ends for the GLYT2 glycine transporter in rat brain DNA by using rapid amplification of cDNA ends (RACE) analysis. Study of the genomic DNA revealed that the isoform diversity is generated by alternative use of exons 1a or 1b, respectively. Upon translation, the mRNA corresponding to the novel isoform GLYT2b would yield a protein five amino acids longer than the previously characterized isoform GLYT2a. Both forms display similar regional distribution and kinetics characteristics. However, whereas GLYT2a is able to actively accumulate glycine into transfected COS cells, GLYT2b seems only to exchange (or release) glycine.
我们通过使用cDNA末端快速扩增（RACE）分析，在大鼠脑DNA中确定了甘氨酸转运体2（GLYT2）的两个替代性5'末端。对基因组DNA的研究表明，亚型多样性分别是由外显子1a或1b的选择性使用产生的。翻译后，对应于新型亚型GLYT2b的mRNA将产生一种比先前鉴定的亚型GLYT2a长五个氨基酸的蛋白质。两种形式都表现出相似的区域分布和动力学特征。然而，虽然GLYT2a能够将甘氨酸主动积累到转染的COS细胞中，但GLYT2b似乎只进行甘氨酸的交换（或释放）。
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