Tsujimoto H, Mukai Y, Akagawa K, Nagaki K, Fujigaki J, Yamamoto M, Sasakuma T
Kihara Institute for Biological Research, Yokohama City University, Japan.
Genes Genet Syst. 1997 Oct;72(5):303-9. doi: 10.1266/ggs.72.303.
The Afa-family repetitive sequences were isolated from barley (Hordeum vulgare, 2n = 14) and cloned as pHvA14. This sequence distinguished each barely chromosome by in situ hybridization. Double color fluorescence in situ hybridization using pHvA14 and 5S rDNA or HvRT-family sequence (subtelomeric sequence of barley) allocated individual barley chromosomes showing a specific pattern of pHvA14 to chromosome 1H to 7H. As the case of the D genome chromosomes of Aegilops squarrosa and common wheat (Triticum aestivum) hybridized by its Afa-family sequences, the signals of pHvA14 in barley chromosomes tended to appear in the distal regions that do not carry many chromosome band markers. In the telomeric regions these signals always placed in more proximal portions than those of HvRT-family. Based on the distribution patterns of Afa-family sequences in the chromosomes of barley and D genome chromosomes of wheat, we discuss a possible mechanism of amplification of the repetitive sequences during the evolution of Triticeae. In addition, we show here that HvRT-family also could be used to distinguish individual barley chromosomes from the patterns of in situ hybridization.
从大麦（Hordeum vulgare，2n = 14）中分离出Afa家族重复序列，并克隆为pHvA14。该序列通过原位杂交区分每条大麦染色体。使用pHvA14和5S rDNA或HvRT家族序列（大麦的亚端粒序列）进行双色荧光原位杂交，可对单个大麦染色体进行定位，显示出pHvA14在1H至7H染色体上的特定模式。如同节节麦和普通小麦（Triticum aestivum）的D基因组染色体通过其Afa家族序列杂交的情况一样，大麦染色体中pHvA14的信号往往出现在不携带许多染色体带标记的远端区域。在端粒区域，这些信号总是比HvRT家族的信号位于更靠近近端的部分。基于Afa家族序列在大麦染色体和小麦D基因组染色体中的分布模式，我们讨论了小麦族进化过程中重复序列扩增的可能机制。此外，我们在此表明，HvRT家族也可用于通过原位杂交模式区分单个大麦染色体。
