Schreiber V, Masson R, Linares J L, Mattei M G, Tomasetto C, Rio M C
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP Strasbourg, France.
Gene. 1998 Jan 30;207(2):171-5. doi: 10.1016/s0378-1119(97)00622-7.
The human Lasp-1 (LIM and SH3 protein) gene was previously identified by differential screening of a breast cancer-derived metastatic lymph node cDNA library. It was located on the q12-q21 region of human chromosome 17 and was shown to be amplified and overexpressed in 12% of breast tumors. Lasp-1 defines a new LIM-protein subfamily, as it associates a C-terminal Src homology 3 (SH3) domain to a N-terminal LIM motif. In this study, the isolation and characterization of the cDNA encoding the mouse Lasp-1 protein are described, and it is shown to be highly conserved with its human counterpart. In addition to the LIM and SH3 domains, both human and mouse Lasp-1 contain an actin-binding domain. The mouse gene was mapped by in situ hybridization to the 11C-11D region of chromosome 11. Northern blot analysis shows that this gene is expressed from 7.5 to 17.5 days post-coitum of mouse embryogenesis and in almost all adult tissues.
人类Lasp-1（LIM和SH3蛋白）基因先前是通过对乳腺癌来源的转移性淋巴结cDNA文库进行差异筛选而鉴定出来的。它位于人类17号染色体的q12-q21区域，在12%的乳腺肿瘤中显示出扩增和过表达。Lasp-1定义了一个新的LIM蛋白亚家族，因为它将一个C端Src同源3（SH3）结构域与一个N端LIM基序相连。在本研究中，描述了编码小鼠Lasp-1蛋白的cDNA的分离和特性，并且显示它与其人类对应物高度保守。除了LIM和SH3结构域之外，人类和小鼠的Lasp-1都含有一个肌动蛋白结合结构域。通过原位杂交将小鼠基因定位到11号染色体的11C-11D区域。Northern印迹分析表明，该基因在小鼠胚胎发育的交配后7.5至17.5天以及几乎所有成年组织中都有表达。
