Lurie I S, Hays P A, Casale J F, Moore J M, Castell D M, Chan K C, Issaq H J
US Drug Enforcement Administration, Special Testing and Research Laboratory, McLean, VA 22102-3494, USA.
Electrophoresis. 1998 Jan;19(1):51-6. doi: 10.1002/elps.1150190110.
The analysis of by-products and impurities in illicit cocaine, including the isomeric truxillines, is important for derivation of both strategic and tactical intelligence. In the present study, various capillary electrophoresis techniques were investigated for this purpose. The use of the anionic beta-cyclodextrin sulfobutyl ether IV as a run buffer additive at pH 8.6 gave a good separation of the truxillines and similar high molecular weight impurities in less than eight minutes. These impurities were first isolated from the bulk cocaine matrix using liquid-liquid extraction and size-exclusion high performance liquid chromatography. There was a red shift in the UV spectra obtained for the truxillines using photodiode array (PDA) UV detection during CE analysis. This anomalous behavior is attributed to photo-degradation of the truxillines during the PDA-UV irradiation process. Laser-induced fluorescence detection using a UV krypton/fluoride laser provided greater selectivity and sensitivity versus UV detection for certain uncharacterized high molecular weight impurities.
对非法可卡因中的副产物和杂质（包括异构古柯碱）进行分析，对于获取战略和战术情报都很重要。在本研究中，为此目的研究了各种毛细管电泳技术。在pH 8.6条件下使用阴离子型磺丁基醚-β-环糊精IV作为运行缓冲添加剂，可在不到八分钟的时间内对古柯碱和类似的高分子量杂质进行良好分离。这些杂质首先使用液液萃取和尺寸排阻高效液相色谱从大量可卡因基质中分离出来。在毛细管电泳分析期间，使用光电二极管阵列（PDA）紫外检测获得的古柯碱紫外光谱出现红移。这种异常行为归因于在PDA-UV照射过程中古柯碱的光降解。对于某些未表征的高分子量杂质，使用紫外氪/氟激光器进行激光诱导荧光检测比紫外检测具有更高的选择性和灵敏度。
