Clatch R J, Foreman J R, Walloch J L
Christ Hospital and Medical Center, Oak Lawn, Illinois, USA.
Cytometry. 1998 Feb 15;34(1):3-16. doi: 10.1002/(sici)1097-0320(19980215)34:1<3::aid-cyto2>3.0.co;2-l.
Immunophenotypic analysis of hematologic specimens is a useful laboratory adjunct to surgical pathology and cytology to confirm or further characterize diagnoses of leukemia or lymphoma. Laser scanning cytometry is a new laboratory technology that has been adapted to perform immunophenotypic analysis of hematologic specimens, with numerous advantages as compared with flow cytometry. In order to make full use of the laser scanning cytometer's capabilities, a new method of specimen preparation and means of performing the immunofluorescent reactions was developed. The technique described in this report, specific only to laser scanning cytometry, enables panels of up to 36 different antibodies to be used on specimens as small as 50,000 total cells. The laboratory methodology is simple, requires 85% less antibody than flow cytometric methods, and allows individual cell cytologic morphology to be correlated with objective physical and fluorescent measurements on a cell-by-cell basis. Other advantages are described in the text. Over the course of nine months in our community hospital, we have used this technique clinically to analyze 172 cases of suspected leukemia or lymphoma. The method has proven remarkably useful, particularly for extremely small specimens such as fine needle aspiration biopsies.
血液学标本的免疫表型分析是外科病理学和细胞学的一项有用的实验室辅助手段，用于确诊或进一步明确白血病或淋巴瘤的诊断。激光扫描细胞术是一种新的实验室技术，已被应用于血液学标本的免疫表型分析，与流式细胞术相比有诸多优势。为了充分利用激光扫描细胞仪的功能，开发了一种新的标本制备方法和进行免疫荧光反应的手段。本报告中描述的技术仅适用于激光扫描细胞术，能够在多达36种不同抗体的组合下，应用于总数仅50,000个细胞的小标本。该实验室方法简单，所需抗体比流式细胞术方法少85%，并允许在逐个细胞的基础上，将单个细胞的细胞学形态与客观的物理和荧光测量结果相关联。文中还描述了其他优势。在我们社区医院的九个月时间里，我们已在临床上使用该技术分析了172例疑似白血病或淋巴瘤病例。该方法已证明非常有用，特别是对于极少量标本，如细针穿刺活检标本。
