Clatch R J, Walloch J L
Department of Pathology, Christ Hospital and Medical Center, Oak Lawn, Illinois 60453, USA.
Acta Cytol. 1997 Jan-Feb;41(1):109-22. doi: 10.1159/000332314.
To test the new laboratory technology of laser scanning cytometry with respect to immunophenotyping of all types of hematologic and lymphoreticular specimens and particularly those of limited size, such as fine needle aspiration biopsies and hypocellular body fluids.
Over the course of two years, 343 hematologic and lymphoreticular specimens of all types were immunophenotyped by laser scanning cytometry using methodologies modified from those of conventional flow cytometric immunophenotyping. Results for all cases were corroborated with histology and/or cytology and, for some cases, immunohistochemistry and/or flow cytometric immunophenotyping.
Over 98% of the 343 cases were successfully immunophenotyped by laser scanning cytometry. These included many hypocellular specimens, such as 38 fine needle aspiration biopsies and 33 body fluid specimens.
Laser scanning cytometry is a new laboratory technology with several significant advantages relative to flow cytometry for immunophenotypic analysis of hematologic malignancy. The laboratory techniques are simplified, and antibody usage is reduced by 80%. Even more important, full-panel immunophenotyping with multiple antibodies can be performed on specimens as small as 50,000 cells total, making the technology particularly relevant to cytopathology. After immunophenotypic analysis, specimens can be stained for light microscopic examination, and individual cells meeting user-defined antigenic or physical characteristics can be automatically relocalized.
针对各类血液学和淋巴网状组织标本，尤其是有限大小的标本，如细针穿刺活检标本和低细胞体液标本，测试激光扫描细胞术这一新型实验室技术用于免疫表型分析的效果。
在两年时间里，采用从传统流式细胞术免疫表型分析方法改良而来的方法，对343份各类血液学和淋巴网状组织标本进行激光扫描细胞术免疫表型分析。所有病例的结果均与组织学和/或细胞学结果进行了对照，部分病例还与免疫组织化学和/或流式细胞术免疫表型分析结果进行了对照。
343例病例中超过98%通过激光扫描细胞术成功进行了免疫表型分析。这些病例包括许多低细胞标本，如38份细针穿刺活检标本和33份体液标本。
对于血液系统恶性肿瘤的免疫表型分析，激光扫描细胞术是一项相对于流式细胞术具有若干显著优势的新型实验室技术。该实验室技术得到简化，抗体用量减少80%。更重要的是，对于总数低至50000个细胞的标本也可进行多种抗体的全组免疫表型分析，这使得该技术与细胞病理学特别相关。免疫表型分析后，标本可进行染色以用于光学显微镜检查，符合用户定义的抗原或物理特征的单个细胞可自动重新定位。
