Kesner J S, Knecht E A, Krieg E F, Wilcox A J, O'Connor J F
Experimental Toxicology Branch, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, Cincinnati, OH 45226, USA.
Hum Reprod. 1998 Jan;13(1):15-21. doi: 10.1093/humrep/13.1.15.
The study objectives were to determine (i) if pre-ovulatory luteinizing hormone (LH) surges, undetected in urine by two immunoradiometric assays (IRMA), were detectable by an ultrasensitive immunofluorometric assay (IFMA) and (ii) the influence of creatinine adjustment on the detection and timing of the urinary LH surges. Daily urine specimens were contributed by healthy 25-36 year old volunteers during 14 ovulatory menstrual cycles for an epidemiological study conducted in 1983-1985. Specimens were selected as having been previously assayed by two IRMA without consistently detecting LH surges. These urine specimens were remeasured using an IFMA and adjusted for creatinine concentration. IFMA measurements revealed unambiguous LH surges in all cycles. Adjusting IRMA urinary LH values for creatinine concentrations revealed previously undetected LH surges in four of eight cycles. Creatinine adjustment also altered the timing of IRMA and IFMA LH surges by 1-5 days. These results demonstrate an IFMA that detects pre-ovulatory LH surges in unpreserved, frozen urine from cycles where such surges were previously undetectable. Further, creatinine adjustment can markedly affect detection and timing of the onset and peak of the urinary LH surge. While our analysis suggests that this adjustment improves the validity of the LH measure, this requires further investigation.
（i）两种免疫放射分析（IRMA）在尿液中未检测到的排卵前促黄体生成素（LH）激增，是否能被超灵敏免疫荧光分析（IFMA）检测到；以及（ii）肌酐校正对尿液LH激增检测及时间的影响。在1983年至1985年进行的一项流行病学研究中，健康的25至36岁志愿者在14个排卵月经周期中每天提供尿液样本。这些样本之前用两种IRMA检测过，但未持续检测到LH激增。使用IFMA对这些尿液样本重新测量，并根据肌酐浓度进行校正。IFMA测量显示所有周期中均有明确的LH激增。根据肌酐浓度校正IRMA尿液LH值后，在八个周期中的四个周期中发现了之前未检测到的LH激增。肌酐校正还使IRMA和IFMA LH激增的时间改变了1至5天。这些结果表明，IFMA能够检测到之前未检测到LH激增的周期中未保存的冷冻尿液中的排卵前LH激增。此外，肌酐校正可显著影响尿液LH激增开始和峰值的检测及时间。虽然我们的分析表明这种校正提高了LH测量的有效性，但这还需要进一步研究。
