Son1p是酿酒酵母26S蛋白酶体的一个组成部分。
Son1p is a component of the 26S proteasome of the yeast Saccharomyces cerevisiae.
作者信息
Fujimuro M, Tanaka K, Yokosawa H, Toh-e A
机构信息
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
出版信息
FEBS Lett. 1998 Feb 20;423(2):149-54. doi: 10.1016/s0014-5793(98)00084-2.
A son1 mutant was isolated as a mutant showing synthetic lethality with nin1-1 which is defective in the p31 component of the regulatory subunit of the yeast 26S proteasome. son1delta showed a synthetic effect with sen3delta and sun1delta, both components of the 26S proteasome, and with cdc28-1N. The 26S proteasome was partially purified from the wild type yeast. The FPLC fractions were analyzed by Western blotting using anti-Son1p antibody and antibodies against some authentic subunits of the 26S proteasome, and we found that Son1p co-migrated with components of the 26S proteasome. The 26S proteasome containing fraction was immunoprecipitated with anti-Son1p antibody. The resultant precipitate contained Nin1p, Sun1p, TBP1, and the 20S proteasome. Combining genetic and biochemical results together, we concluded that Son1p is a component of the yeast 26S proteasome.
分离到一个son1突变体，该突变体与nin1 - 1表现出合成致死性，nin1 - 1在酵母26S蛋白酶体调节亚基的p31成分中存在缺陷。son1δ与26S蛋白酶体的两个成分sen3δ和sun1δ以及cdc28 - 1N表现出合成效应。从野生型酵母中部分纯化了26S蛋白酶体。使用抗Son1p抗体和针对26S蛋白酶体一些真实亚基的抗体，通过蛋白质免疫印迹法分析快速蛋白质液相色谱（FPLC）级分，我们发现Son1p与26S蛋白酶体的成分共迁移。用抗Son1p抗体对含有26S蛋白酶体的级分进行免疫沉淀。所得沉淀物包含Nin1p、Sun1p、TBP1和20S蛋白酶体。综合遗传和生化结果，我们得出结论，Son1p是酵母26S蛋白酶体的一个成分。
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