芽殖酵母中26S蛋白酶体的生长依赖性变化。

Growth-dependent change of the 26S proteasome in budding yeast.

作者信息

Fujimuro M, Takada H, Saeki Y, Toh-e A, Tanaka K, Yokosawa H

机构信息

Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060-0812, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Oct 29;251(3):818-23. doi: 10.1006/bbrc.1998.9560.

DOI:10.1006/bbrc.1998.9560

PMID:9790993

Abstract

The 26S proteasome is assembled from the 20S proteasome and the regulatory subunit complex in an ATP-dependent manner. In the present study, we found that the ATP-dependent activity and the protein amount of the 26S proteasome change during growth of the budding yeast Saccharomyces cerevisiae. Both levels in the stationary phase are higher than those in the exponentially growing phase. On the other hand, the levels of the 20S proteasome appear to remain unchanged during growth. These results suggest that the 26S proteasome undergoes a growth-dependent change and that the 26S proteasome plays a role in the survival of yeast cells under starvation conditions.

摘要

26S蛋白酶体以ATP依赖的方式由20S蛋白酶体和调节亚基复合体组装而成。在本研究中，我们发现26S蛋白酶体的ATP依赖活性和蛋白量在芽殖酵母酿酒酵母生长过程中会发生变化。稳定期的这两个水平均高于指数生长期。另一方面，20S蛋白酶体的水平在生长过程中似乎保持不变。这些结果表明，26S蛋白酶体经历了生长依赖性变化，并且26S蛋白酶体在饥饿条件下酵母细胞的存活中发挥作用。