鸡25-羟基维生素D3 24-羟化酶cDNA的克隆与表达

Cloning and expression of the chicken 25-hydroxyvitamin D3 24-hydroxylase cDNA.

作者信息

Jehan F, Ismail R, Hanson K, DeLuca H F

机构信息

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706, USA.

出版信息

Biochim Biophys Acta. 1998 Feb 11;1395(3):259-65. doi: 10.1016/s0167-4781(97)00177-2.

DOI:10.1016/s0167-4781(97)00177-2

PMID:9512657

Abstract

Using a cDNA probe from the rat 24-hydrovitamin D3 24-hydroxylase, the chicken 25-hydroxyvitamin D3 24-hydroxylase cDNA has been isolated from a chicken kidney lambda gt11 library. The high degree of similarity with the mammalian 24-hydroxylase cDNAs strongly supports the belief that it is the chicken 25-hydroxyvitamin D3 24-hydroxylase cDNA. The deduced amino acid sequences are also very well conserved and 325 of them are identical among the four known 25-hydroxyvitamin D3 24-hydroxylases. This cDNA expressed in E. coli produces 24-hydroxylase activity.

摘要

利用大鼠24-羟维生素D3 24-羟化酶的cDNA探针，从鸡肾λgt11文库中分离出鸡25-羟维生素D3 24-羟化酶的cDNA。与哺乳动物24-羟化酶cDNA的高度相似性有力地支持了这是鸡25-羟维生素D3 24-羟化酶cDNA的观点。推导的氨基酸序列也高度保守，在四种已知的25-羟维生素D3 24-羟化酶中，有325个氨基酸是相同的。在大肠杆菌中表达的这种cDNA具有24-羟化酶活性。