Ishiguro T, Naito M, Hanaoka K, Nagawa H, Muto T, Tsuruo T
First Department of Surgery, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.
Clin Exp Metastasis. 1998 Feb;16(2):179-83. doi: 10.1023/a:1006528422244.
B16-F10 is a B16 mouse melanoma subline that preferentially metastasizes to the lung following intravenous injection. Previously we isolated TI-241 (LRF-1 homologue related to Jun-Fos) gene that was expressed higher in the high metastatic clone B16-F10 than the low metastatic clone F1. Transfection of TI-241 into F1 converted it into a high-metastatic cell. We studied the effect of antisense oligonucleotide designed to reduce the expression of TI-241 in B16-F10 cells, and observed an unexpected increase in the TI-241 level. The increase in the expression was maximal at 30 h, then it decreased during further culture with or without TI-241 antisense oligonucleotide. This increased TI-241 expression by antisense oligonucleotide was also observed in B16-F1 cells whereas sense oligonucleotide did not affect the expression. B16-F10 cells cultured with TI-241 antisense oligonucleotide showed enhanced experimental metastatic potential to the mouse lungs compared with untreated B16-F10 and B16-F10 cultured with TI-241 sense oligonucleotide.
B16-F10是一种B16小鼠黑色素瘤亚系，静脉注射后优先转移至肺部。此前我们分离出TI-241（与Jun-Fos相关的LRF-1同源物）基因，该基因在高转移克隆B16-F10中的表达高于低转移克隆F1。将TI-241转染到F1中可使其转变为高转移细胞。我们研究了旨在降低B16-F10细胞中TI-241表达的反义寡核苷酸的作用，并观察到TI-241水平意外升高。表达增加在30小时时达到最大值，然后在进一步培养过程中，无论有无TI-241反义寡核苷酸，其水平均下降。在B16-F1细胞中也观察到反义寡核苷酸使TI-241表达增加，而正义寡核苷酸不影响其表达。与未处理的B16-F10以及用TI-241正义寡核苷酸培养的B16-F10相比，用TI-241反义寡核苷酸培养的B16-F10细胞对小鼠肺部的实验性转移潜能增强。
