Calder K M, Horwitz M A
Department of Medicine, School of Medicine, University of California, Los Angeles, California, 90095, USA.
Microb Pathog. 1998 Mar;24(3):133-43. doi: 10.1006/mpat.1997.9999.
Iron plays a central role in the pathogenesis of Mycobacterium tuberculosis, the principal causative agent of tuberculosis. To learn more about iron acquisition by this bacterium, its iron regulated proteins (IRPs) were investigated. Seven IRPs were identified - three increased by high iron concentrations, and four by low iron concentrations. The smallest protein induced by low iron, Irp10, is tightly iron regulated as it is virtually absent in bacteria cultured in the presence of high iron concentrations. The gene (irpA ) encoding this protein and an adjacent open reading frame, mtaA, were cloned and sequenced. The protein encoded by mtaA (Mta72) has striking homology to metal transporting P-type ATPases. This study suggests that Irp10 and Mta72 function as a two-component metal transport system in M. tuberculosis.
铁在结核病的主要病原体结核分枝杆菌的发病机制中起着核心作用。为了更多地了解这种细菌获取铁的情况，对其铁调节蛋白（IRP）进行了研究。鉴定出了七种IRP——三种在高铁浓度下增加，四种在低铁浓度下增加。由低铁诱导产生的最小蛋白Irp10受到严格的铁调节，因为在高铁浓度下培养的细菌中几乎不存在该蛋白。编码此蛋白的基因（irpA）和一个相邻的开放阅读框mtaA被克隆并测序。mtaA编码的蛋白（Mta72）与金属转运P型ATP酶具有显著的同源性。这项研究表明，Irp10和Mta72在结核分枝杆菌中作为一个双组分金属转运系统发挥作用。
