Kimura K, Kimura H, Yokosawa N, Isogai H, Isogai E, Kozaki S, Miyamoto A, Nishikawa T, Ohshika H, Kubota T, Fujii N
Department of Microbiology, School of Medicine, Sapporo Medical University, Japan.
Biochem Biophys Res Commun. 1998 Mar 6;244(1):275-9. doi: 10.1006/bbrc.1998.8188.
We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 micrograms/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.
我们证明肉毒杆菌神经毒素可降低培养的心肌细胞的自发搏动率。原代培养的心肌细胞取自新生Wistar大鼠（1 - 3日龄）的心室。在细胞接种7天后，将掺入脂质体的A型肉毒杆菌毒素添加到培养基中。终浓度为5.0微克/毫升时，肉毒杆菌毒素在2 - 4小时内显著降低了心肌细胞的搏动率。这些结果证明了SNARE复合蛋白对心肌细胞自发搏动的作用。
