Maiti I B, Richins R D, Shepherd R J
Department of Plant Pathology, Tobacco and Health Research Institute, University of Kentucky, Lexington 40546-0236, USA.
Virus Res. 1998 Oct;57(2):113-24. doi: 10.1016/s0168-1702(98)00088-4.
Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants. Transgenic plants containing a dicistronic transcription unit (PClSV-gene VII-GUS) under the control of PClSV full-length transcript promoter with its long leader sequence show a relatively high abundance of the expected transcript but very little, or no, GUS activity. However, high GUS activity is found when gene VI protein is then provided by subsequent infection with PClSV. The efficient translation of polycistronic mRNAs mediated by gene VI of caulimovirus has potential value in product engineering of plants.
我们在此证明，花生褪绿条纹病毒（PClSV）的基因VI产物可反式激活双顺反子转录本的翻译，该病毒是该病毒组一个新鉴定的成员。双顺反子表达单元已在原生质体瞬时表达实验和转基因烟草植株中进行了分析。含有在PClSV全长转录本启动子及其长前导序列控制下的双顺反子转录单元（PClSV-基因VII-GUS）的转基因植株，显示出预期转录本的相对高丰度，但GUS活性很低或没有。然而，当随后用PClSV感染提供基因VI蛋白时，可发现高GUS活性。花椰菜花叶病毒基因VI介导的多顺反子mRNA的有效翻译在植物产品工程中具有潜在价值。
