Zeng Q, Hong W, Tan Y H
Institute of Molecular and Cell Biology, Singapore, Singapore.
Biochem Biophys Res Commun. 1998 Mar 17;244(2):421-7. doi: 10.1006/bbrc.1998.8291.
Protein tyrosine phosphatases (PTPs) play a fundamental role in regulating diverse cellular processes. PRL-1 is a unique nuclear PTP that is induced in mitogen-stimulated cells and regenerating liver. Database searches using the PRL-1 sequence led to the identification of mouse PRL-2 and PRL-3 which exhibit 87% and 76% identity to mouse PRL-1 in their amino acid sequences. All three mouse PRL proteins contain a C-terminal consensus sequence for prenylation. All PRL proteins bear significant sequence homology to Cdc14p and the recently identified tumor suppressor PTEN/MMAC1, in regions other than the conserved PTP signature motif. The nucleotide sequences of the coding regions of mouse PRL-2 and PRL-3 are, respectively, 71% and 62%, identical to mouse PRL-1, while the 5' un-translated regions of mouse PRL-1, PRL-2, and PRL-3 are much more divergent. Northern blot analysis revealed that PRL-2 is preferentially expressed in skeletal muscle, while PRL-3 is preferentially expressed in both skeletal muscle and heart, although both PRL-2 and PRL-3 are expressed at lower levels in other tissues.
蛋白质酪氨酸磷酸酶（PTPs）在调节多种细胞过程中发挥着重要作用。PRL-1是一种独特的核PTP，在有丝分裂原刺激的细胞和再生肝脏中被诱导表达。使用PRL-1序列进行数据库搜索，鉴定出了小鼠PRL-2和PRL-3，它们的氨基酸序列与小鼠PRL-1的同一性分别为87%和76%。所有三种小鼠PRL蛋白都含有一个C末端异戊二烯化共有序列。在保守的PTP特征基序以外的区域，所有PRL蛋白与Cdc14p和最近鉴定的肿瘤抑制因子PTEN/MMAC1都具有显著的序列同源性。小鼠PRL-2和PRL-3编码区的核苷酸序列分别与小鼠PRL-1有71%和62%的同一性，而小鼠PRL-1、PRL-2和PRL-3的5'非翻译区差异更大。Northern印迹分析显示，PRL-2在骨骼肌中优先表达，而PRL-3在骨骼肌和心脏中都优先表达，尽管PRL-2和PRL-3在其他组织中的表达水平较低。
