Morris J A, Stevens A E
Res Vet Sci. 1976 Jul;21(1):117-8.
Exhaustive lipid extraction of the Johne's bacilli before preparing the Maltaner-Wadsworth antigen removed both the anticomplementary activity of the preparation and its ability to fix complement. Gel filtration temporarily removed anticomplementary activity but tended to reduce the antigenic activity of the extract. Sonication significantly reduced the anticomplementary activity of the antigen without affecting its capacity to fix complement but the effects were only temporary. It is suggested that the antigen exists as micelles which gradually aggregate and re-arrange thereby exposing anticomplementary sites hitherto masked in the dispersed micelles.
在制备马尔塔纳-沃兹沃思抗原之前，对约内氏杆菌进行彻底的脂质提取，去除了制剂的抗补体活性及其固定补体的能力。凝胶过滤暂时去除了抗补体活性，但往往会降低提取物的抗原活性。超声处理显著降低了抗原的抗补体活性，而不影响其固定补体的能力，但效果只是暂时的。有人认为，抗原以微团形式存在，这些微团会逐渐聚集和重新排列，从而暴露出迄今隐藏在分散微团中的抗补体位点。
