Herr S, Huchzermeyer H F, Te Brugge L A, Williamson C C, Roos J A, Schiele G J
Onderstepoort J Vet Res. 1985 Dec;52(4):279-82.
The same techniques may be used in the complement fixation test (CFT) for the serological diagnosis of bovine brucellosis, Johne's disease (paratuberculosis), dourine, equine piroplasmosis and Q fever (caused by Coxiella burnetii). The reproducibility of results is excellent, falling for the most part within the twofold range and never exceeding the fourfold range. Agreement with other laboratories is excellent (i.e. within twofold) in the case of brucellosis and equine piroplasmosis antibody titres. A good correlation between the occurrence of the disease and serological reactions is found on circumstantial evidence in the cases of dourine, Johne's disease and Q fever. A standard unitage system is used to report the antibody titres found in all the tests. To simplify laboratory protocols, laboratories required to employ the CFT for the diagnosis of these diseases are advised to use a single proven technique in all the tests. Problems experienced with transient false-positive Johne's disease antibody titres in cattle following on tuberculin (bovine and avian) testing make it advisable to take specimens for the Johne's disease test prior to performing the tuberculin tests.
相同的技术可用于补体结合试验（CFT），以进行牛布鲁氏菌病、副结核病（约翰氏病）、马媾疫、马梨形虫病和Q热（由伯氏考克斯氏体引起）的血清学诊断。结果的重现性极佳，大部分结果落在两倍范围内，且从未超过四倍范围。在布鲁氏菌病和马梨形虫病抗体效价方面，与其他实验室的一致性极佳（即两倍范围内）。在马媾疫、约翰氏病和Q热病例中，根据间接证据发现疾病的发生与血清学反应之间存在良好的相关性。所有检测中发现的抗体效价均采用标准单位体系报告。为简化实验室规程，建议要求使用CFT诊断这些疾病的实验室在所有检测中采用单一经过验证的技术。牛在进行结核菌素（牛型和禽型）检测后出现约翰氏病抗体效价短暂假阳性的问题，因此建议在进行结核菌素检测之前采集用于约翰氏病检测的样本。
