Fokkens W J, Vinke J G, De Jong S S, Bogaert D P, Kleinjan A, Eichhorn E
Department of Otorhinolaryngology, Erasmus University Medical Centre Rotterdam, The Netherlands.
Clin Exp Allergy. 1998 Feb;28(2):187-95. doi: 10.1046/j.1365-2222.1998.00209.x.
Allergic sensitization of the airways occurs in the mucosa of the shock organ, or in the lymphatic stations draining these structures. The lymphatic structure closest to the nasal mucosa is the adenoid.
The objective of this study was to find evidence for our hypothesis that allergic sensitization can occur in the adenoid. Of special interest, in this context are cell types involved in antigen-allergen presentation (e.g. Langerhans cells) and effector cells of allergic disease.
In this study cellular infiltrates in adenoids of 16 allergic patients and 16 age- and gender-matched controls were evaluated. The number of cells positive for CD1a, CD4, CD8, CD-68, chymase, tryptase, IgE, MBP and cells positive for interleukin (IL)-4 were determined using immunohistochemical staining techniques. The epithelium, follicles and the interfollicular spaces were evaluated separately.
When comparing the two groups a significant increase in cells positive for CD1a was found in interfollicular spaces of the allergic group (P = 0.008). CD1a+ cells in the follicular space and eosinophils in the interfollicular space showed a trend to be more numerous in the allergic group (P = 0.02 and P = 0.05, respectively). The other cell types investigated did not show significant differences between the groups.
The results of this study show for the first time that cells involved in allergic sensitization and allergic disease differ in the adenoid of allergic children compared with controls. These findings support our hypothesis that allergic sensitization takes place in the adenoid. Furthermore, this study confirms that CD1a+ (Langerhans) cells are involved in allergic disease.
气道的过敏性致敏发生在休克器官的黏膜或引流这些结构的淋巴站点。最靠近鼻黏膜的淋巴结构是腺样体。
本研究的目的是为我们的假设寻找证据，即过敏性致敏可发生在腺样体中。在这种情况下，特别令人感兴趣的是参与抗原-变应原呈递的细胞类型（如朗格汉斯细胞）和过敏性疾病的效应细胞。
在本研究中，对16例过敏患者和16例年龄及性别匹配的对照者的腺样体中的细胞浸润情况进行了评估。使用免疫组织化学染色技术确定CD1a、CD4、CD8、CD-68、糜酶、类胰蛋白酶、IgE、髓过氧化物酶（MBP）阳性细胞以及白细胞介素（IL）-4阳性细胞的数量。分别对上皮、滤泡和滤泡间区域进行评估。
比较两组时，发现过敏组滤泡间区域中CD1a阳性细胞显著增加（P = 0.008）。滤泡区域中的CD1a+细胞和滤泡间区域中的嗜酸性粒细胞在过敏组中显示出数量更多的趋势（分别为P = 0.02和P = 0.05）。所研究的其他细胞类型在两组之间未显示出显著差异。
本研究结果首次表明，与对照组相比，过敏性儿童腺样体中参与过敏性致敏和过敏性疾病的细胞存在差异。这些发现支持了我们关于过敏性致敏发生在腺样体中的假设。此外，本研究证实CD1a+（朗格汉斯）细胞参与过敏性疾病。
