Masure H R, Pearce B J, Shio H, Spellerberg B
Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105-2794, USA.
Mol Microbiol. 1998 Feb;27(4):845-52. doi: 10.1046/j.1365-2958.1998.00732.x.
Recombination in prokaryotes and eukaryotes is mediated by the RecA family of proteins. Although the interactions between RecA and DNA are well studied, the cellular location of these interactions is not known. Using genetic transformation of Streptococcus pneumoniae as a model system, there was increased expression of a protein, colligrin, and RecA, products of the rec locus during genetic transfer. These proteins formed a complex and were found associated with the membranes of genetically competent cells. With immunoelectron microscopy and subcellular fractionation, we showed that the induction of competence led to the translocation of RecA and colligrin to the membrane and to the formation of clusters of RecA in a colligrin-dependent step. Based on the behaviour of colligrin and RecA during genetic exchange and the numerous proteins in prokaryotes and eukaryotes with domains similar to colligrin, we suggest that there may exist a family of proteins, which gathers macromolecules at specific sites in biological membranes.
原核生物和真核生物中的重组由RecA蛋白家族介导。尽管RecA与DNA之间的相互作用已得到充分研究，但这些相互作用在细胞内的位置尚不清楚。以肺炎链球菌的基因转化作为模型系统，在基因转移过程中，rec基因座的产物——一种名为colligrin的蛋白质和RecA的表达增加。这些蛋白质形成复合物，并被发现与感受态细胞的膜相关联。通过免疫电子显微镜和亚细胞分级分离，我们表明感受态的诱导导致RecA和colligrin转运至膜上，并在一个依赖colligrin的步骤中形成RecA簇。基于colligrin和RecA在基因交换过程中的行为以及原核生物和真核生物中许多具有与colligrin相似结构域的蛋白质，我们推测可能存在一类蛋白质家族，它们在生物膜的特定位点聚集大分子。
