感官视紫红质II转导蛋白HtrII也负责古菌盐生盐杆菌中的丝氨酸趋化作用。
Sensory rhodopsin II transducer HtrII is also responsible for serine chemotaxis in the archaeon Halobacterium salinarum.
作者信息
Hou S, Brooun A, Yu H S, Freitas T, Alam M
机构信息
Department of Microbiology, University of Hawaii, Honolulu 96822, USA.
出版信息
J Bacteriol. 1998 Mar;180(6):1600-2. doi: 10.1128/JB.180.6.1600-1602.1998.
Abstract
Previously, we demonstrated that the methyl-accepting protein HtrII is the transducer for photoreceptor sensory rhodopsin II. Here, we provide experimental evidence that HtrII is also a chemotransducer. Using an agarose-in-plug bridge method, we show that an HtrII overexpression strain has a quicker response to serine than does an HtrII deletion strain. Furthermore, an in vivo flow assay demonstrates that the deletion strain is unable to modulate methylesterase activity after serine addition or photostimulation, while the overexpression strain shows distinct methanol peaks following both types of stimuli.
摘要
此前，我们证明了甲基接受蛋白HtrII是光感受器感觉视紫红质II的转导器。在此，我们提供实验证据表明HtrII也是一种化学转导器。使用琼脂糖包埋桥法，我们发现HtrII过表达菌株对丝氨酸的反应比HtrII缺失菌株更快。此外，体内流动分析表明，缺失菌株在添加丝氨酸或光刺激后无法调节甲酯酶活性，而过表达菌株在两种刺激后均显示出明显的甲醇峰。
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