Yamashiro D J, Liu X G, Lee C P, Nakagawara A, Ikegaki N, McGregor L M, Baylin S B, Brodeur G M
Division of Oncology, Children's Hospital of Philadelphia, Pennsylvania 19104-4318, USA.
Eur J Cancer. 1997 Oct;33(12):2054-7. doi: 10.1016/s0959-8049(97)00309-2.
Human neuroblastomas express the neurotrophin receptors trk-A and trk-B. Favourable outcome is associated with expression of trk-A, while unfavourable, MYCN amplified tumours express trk-B. In this study we examined the expression of trk-C in primary neuroblastoma tumour-derived cell lines. We found by Northern blot analysis that trk-C mRNA is expressed in 14 of 55 (25%) primary tumours. Trk-C was expressed in significantly more lower stage tumours (stage 1, 2, 4S) than higher stage tumours (stage 3, 4, P < 0.04). The expression of trk-C was correlated positively with survival and negatively correlated with MYCN amplification. We also studied the function of trk-C in transfected cell lines and found that NT-3 promotes both cell survival and differentiation. Our results suggest that trk-C is involved in the biology of favourable neuroblastomas.
人类神经母细胞瘤表达神经营养因子受体trk-A和trk-B。良好的预后与trk-A的表达相关，而预后不良的MYCN扩增肿瘤则表达trk-B。在本研究中，我们检测了原发性神经母细胞瘤肿瘤衍生细胞系中trk-C的表达。通过Northern印迹分析，我们发现55个原发性肿瘤中有14个（25%）表达trk-C mRNA。与晚期肿瘤（3期、4期）相比，trk-C在显著更多的早期肿瘤（1期、2期、4S期）中表达（P < 0.04）。trk-C的表达与生存率呈正相关，与MYCN扩增呈负相关。我们还研究了trk-C在转染细胞系中的功能，发现NT-3可促进细胞存活和分化。我们的结果表明，trk-C参与了预后良好的神经母细胞瘤的生物学过程。
