分枝杆菌属的溶血活性
Haemolytic activity of Mycobacterium spp.
作者信息
Brzychcy M, Andrzejczyk Z, Zalewska N, Zwolska Z, Rudnicka W
机构信息
Department of Infectious Biology, University of Lódź, Poland.
出版信息
Acta Microbiol Pol. 1997;46(4):377-85.
Haemolytic activity of clinical isolates of Mycobacterium bacilli (98) was determined by the method of King et al., 1993. During 3-h incubation, all M. tuberculosis (MTB) isolates (28) and one out of 38 M. avium-intracellulare (MAI) strains, produced a strong contact-dependent haemolysin (CDH). Six MAI strains expressed a weak CDH. One MAI isolate produced a strong and five other MAI strains a weak contact-independent haemolysin (CIH). Two M. bovis BCG strains and 7 M. vaccae strains did not demonstrate haemolytic activity. The persistence of chosen Mycobacterium strains differing by haemolytic activity, in the spleens of infected C57BL/6 mice was examined. Mycobacteria producing a strong CDH (MTB H37Rv, MTB 101/92, MAI 83/93) or CIH (MAI 475/93) survived in the spleens of nonimmunized or M. bovis BCG-immunized mice for longer time than MAI strains expressing weak haemolytic activity or M. bovis BCG vaccine strain.
采用金氏等人1993年的方法测定了98株分枝杆菌临床分离株的溶血活性。在3小时的孵育过程中，所有结核分枝杆菌（MTB）分离株（28株）以及38株鸟分枝杆菌-胞内分枝杆菌（MAI）菌株中的1株产生了强烈的接触依赖性溶血素（CDH）。6株MAI菌株表达弱CDH。1株MAI分离株产生强烈的接触非依赖性溶血素（CIH），其他5株MAI菌株产生弱的接触非依赖性溶血素。2株牛分枝杆菌卡介苗菌株和7株母牛分枝杆菌菌株未表现出溶血活性。研究了溶血活性不同的选定分枝杆菌菌株在感染的C57BL/6小鼠脾脏中的持续性。产生强烈CDH（MTB H37Rv、MTB 101/92、MAI 83/93）或CIH（MAI 475/93）的分枝杆菌在未免疫或牛分枝杆菌卡介苗免疫小鼠的脾脏中存活的时间比表达弱溶血活性的MAI菌株或牛分枝杆菌卡介苗疫苗株更长。
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