da Costa C C, van der Laan L J, Dijkstra C D, Brück W
Department of Neuropathology, University of Göttingen, Germany.
Eur J Neurosci. 1997 Dec;9(12):2650-7. doi: 10.1111/j.1460-9568.1997.tb01694.x.
Myelin phagocytosis during Wallerian degeneration and immune-mediated demyelination depends on the action of mononuclear cells of the monocyte/macrophage system. The present study investigated the role of the macrophage scavenger receptor, a trimeric membrane glycoprotein, in myelin uptake by macrophages. Two in vitro models of myelin phagocytosis were used: an organ culture model of mouse peripheral nerves exposed to cocultured macrophages and a quantitative flow cytometric assay. Different concentrations of the monoclonal rat anti-mouse scavenger receptor antibody (2F8) were applied to these systems to selectively block the macrophage scavenger receptor. Concentration-dependent effects on macrophage migration and myelin uptake were seen when the macrophage scavenger receptor was blocked by the antibody 2F8. Low concentrations reduced myelin phagocytosis by the invading macrophages; higher concentrations completely abolished macrophage invasion of the nerves. Using a quantitative flow cytometric assay it was also shown that the 2F8 antibody inhibits phagocytosis of myelin in a dose-dependent manner. These data indicate that the macrophage scavenger receptor is involved in myelin phagocytosis by macrophages.
沃勒变性和免疫介导的脱髓鞘过程中的髓鞘吞噬作用取决于单核细胞/巨噬细胞系统中单核细胞的作用。本研究调查了巨噬细胞清道夫受体（一种三聚体膜糖蛋白）在巨噬细胞摄取髓鞘中的作用。使用了两种髓鞘吞噬作用的体外模型：暴露于共培养巨噬细胞的小鼠周围神经器官培养模型和定量流式细胞术检测法。将不同浓度的单克隆大鼠抗小鼠清道夫受体抗体（2F8）应用于这些系统，以选择性阻断巨噬细胞清道夫受体。当抗体2F8阻断巨噬细胞清道夫受体时，观察到对巨噬细胞迁移和髓鞘摄取的浓度依赖性影响。低浓度降低了侵入性巨噬细胞的髓鞘吞噬作用；高浓度则完全消除了巨噬细胞对神经的侵入。使用定量流式细胞术检测法还表明，2F8抗体以剂量依赖性方式抑制髓鞘的吞噬作用。这些数据表明巨噬细胞清道夫受体参与了巨噬细胞的髓鞘吞噬作用。
