Bragina E E
Tsitologiia. 1976 Mar;18(3):344-6.
Neoplastic fibroblasts of L strain were studied 24 hours after attachment to the solid artifical substrate. The cells were oriented on sections perpendicular to the substrate plane. Transformed fibroblasts moved forward 24 hours after attachment, being polarized. The main difference between normal fibroblasts studied earlier and the transformed ones was observed in the area of the spread lamellar cytoplasm on the front edge of the cell. In this place in malignant cells, a diminition of size and number of bundles of microfilaments and zones of close junction with the substrate was seen. These elements lie close to each other in normal cells, and far from each other in malignant ones. Another difference between normal and transformed cells is that the latter have many microvilli in the lower part of the surface of lamellar cytoplasm. Ultrastructural differences between normal neoplasmic cells observed in this work may be the cause of the behaviour of neoplastic cells in tissue culture.
对L株肿瘤成纤维细胞附着于固体人工基质24小时后的情况进行了研究。细胞在垂直于基质平面的切片上呈定向排列。转化后的成纤维细胞在附着24小时后向前移动，呈极化状态。早期研究的正常成纤维细胞与转化后的成纤维细胞之间的主要差异，在细胞前缘展开的片状细胞质区域观察到。在恶性细胞的这个部位，可以看到微丝束的大小和数量以及与基质紧密连接的区域有所减少。这些成分在正常细胞中彼此靠近，而在恶性细胞中彼此远离。正常细胞与转化细胞的另一个区别是，后者在片状细胞质表面的下部有许多微绒毛。在这项研究中观察到的正常肿瘤细胞之间的超微结构差异，可能是肿瘤细胞在组织培养中行为表现的原因。
