Shuklinov V A, Livshits V L, Shumilina V V, Tregubova N A, Isakova L M
Tsitologiia. 1980 Jul;22(7):804-9.
In a medium supplemented with 60 per cent large cattle serum, part of the cell population of L-transformed culture was seen to be differentiated into mature lipocytes. The formation of structures similar to normal fat tissue took place in the same conditions, provided a reduced quantity of the seeded cells was used. The cell differentiation was accompanied with a decrease in acid phosphatase and non-specific esterase activities. The ultrastructural study has demonstrated a suitable preservation of cytoplasmic structures and a domination of the rough cytoplasmic reticulum compared to the control cell cultures where free ribosomes and polysomes predominated.
在添加了60%大牛血清的培养基中，L转化培养物的部分细胞群体可分化为成熟脂肪细胞。在相同条件下，只要接种的细胞数量减少，就会形成类似于正常脂肪组织的结构。细胞分化伴随着酸性磷酸酶和非特异性酯酶活性的降低。超微结构研究表明，与以游离核糖体和多聚核糖体为主的对照细胞培养物相比，细胞质结构保存良好，粗面内质网占优势。
