van Valburg A A, van Roy H L, Lafeber F P, Bijlsma J W
Department of Rheumatology and Clinical Immunology, University Hospital, Utrecht, The Netherlands.
J Rheumatol. 1998 Mar;25(3):515-20.
To investigate the in vitro effects of low physiological levels of intermittent fluid pressure (0-13 kPa; 0.33 Hz), in the absence of mechanical stress, on articular cartilage, inflammatory cells, and on the combination of these components, present in the osteoarthritic (OA) joint.
Normal and OA cartilage was obtained at autopsy. Mononuclear cells (MNC) were isolated from synovial fluid of patients with osteoarthritis (OA-SF) or peripheral blood from healthy donors (PB). Cartilage and MNC were cultured alone and in co-culture; cultures were carried out with and without exposure to intermittent fluid pressure. Levels of intermittent fluid pressure varied from 0 to 13 kPa (0.33 Hz), comparable with levels present during joint distraction in treatment of OA. Changes in cartilage proteoglycan metabolism and MNC cytokine production were analyzed.
Cartilage matrix synthesis was stimulated by intermittent fluid pressure in OA cartilage, while normal cartilage was not affected. No effects on proteoglycan release were detected. Inhibition of cartilage proteoglycan synthesis, induced by OA synovial fluid MNC (OA-SF-MNC) in co-culture, was reduced when cultures had been exposed to intermittent fluid pressure. Analysis of conditioned media of OA-SF-MNC revealed that the beneficial effect of intermittent fluid pressure was accompanied by a decrease in production of the catabolic cytokines interleukin 1 and tumor necrosis factor-alpha. Similar effects were observed for MNC isolated from PB.
Low levels of intermittent fluid pressure, as occur in vivo during joint distraction, have beneficial effects on joint tissue in OA, indicating that this factor could be useful in treatment of OA.
在不存在机械应力的情况下，研究低生理水平的间歇性流体压力（0 - 13千帕；0.33赫兹）对骨关节炎（OA）关节中存在的关节软骨、炎症细胞以及这些成分组合的体外影响。
在尸检时获取正常和OA软骨。从骨关节炎患者的滑液（OA - SF）或健康供体的外周血（PB）中分离单核细胞（MNC）。软骨和MNC单独培养以及共培养；培养在有和没有暴露于间歇性流体压力的情况下进行。间歇性流体压力水平从0到13千帕（0.33赫兹）变化，与OA治疗中关节牵张期间存在的水平相当。分析软骨蛋白聚糖代谢和MNC细胞因子产生的变化。
间歇性流体压力刺激OA软骨中的软骨基质合成，而正常软骨不受影响。未检测到对蛋白聚糖释放的影响。当培养物暴露于间歇性流体压力时，共培养中由OA滑液MNC（OA - SF - MNC）诱导的软骨蛋白聚糖合成抑制作用降低。对OA - SF - MNC的条件培养基分析表明，间歇性流体压力的有益作用伴随着分解代谢细胞因子白细胞介素1和肿瘤坏死因子 - α产生的减少。从PB分离的MNC也观察到类似效果。
关节牵张期间体内出现的低水平间歇性流体压力对OA关节组织具有有益作用，表明该因素可能对OA治疗有用。
