木瓜凝乳蛋白酶诱导膝关节损伤后，抗原性硫酸角质素和透明质酸血液水平升高的区域差异。

Regional differences in the rise in blood levels of antigenic keratan sulfate and hyaluronan after chymopapain induced knee joint injury.

作者信息

Rayan V, Thonar E J, Chen L M, Lenz M E, Williams J M

机构信息

Department of Anatomy, Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA.

出版信息

J Rheumatol. 1998 Mar;25(3):521-6.

PMID:9517774

Abstract

OBJECTIVE

Results from several recent studies suggest that the levels of antigenic keratan sulfate (agKS) and hyaluronan (HA) in serum provide useful information about changes taking place in injured or diseased synovial joints. To improve our understanding of the significance of such changes, we investigated the points of entry of these molecules into the blood circulation and their subsequent clearance after experimentally induced injury to rabbit knee joint.

METHODS

Chymopapain was injected into knee joints of 8 young adult rabbits to induce aggrecan degradation in articular cartilage within the injected joint. Levels of agKS and HA in serum from various blood vessels were measured before and 5 h after the injury. The statistical significance of injury related changes and differences among the different vessels were evaluated.

RESULTS

After the injury, the level of agKS rose most significantly in the popliteal vein draining the injected knee joint and dropped rapidly by the time the blood reached the femoral vein. The level of agKS was similar, although lower, in other blood vessels but, in each case, it was significantly higher than before the injection. The level of HA showed a different pattern of changes after injection. While highest in the popliteal vein draining the injected knee, HA was markedly elevated in the cranial vena cava, close to the entry of lymph into the circulation, and was 50% lower in the hepatic than in the portal vein.

CONCLUSION

(1) Measurement of agKS and HA in a blood vessel draining or close to an injured/diseased knee joint may provide more specific information about degradative changes taking place in that joint than measurement of levels of these markers in other blood vessels; (2) some HA molecules but no measurable amounts of agKS enter the blood circulation via the lymphatic system: and (3) HA but not agKS is very rapidly cleared from the blood by the liver.

摘要

目的

近期多项研究结果表明，血清中抗原性硫酸角质素（agKS）和透明质酸（HA）的水平可为了解受损或患病滑膜关节的变化提供有用信息。为了更好地理解这些变化的意义，我们研究了这些分子进入血液循环的途径以及实验性诱导兔膝关节损伤后它们随后的清除情况。

方法

向8只成年幼兔的膝关节注射木瓜凝乳蛋白酶，以诱导注射关节内的关节软骨中聚集蛋白聚糖降解。在损伤前及损伤后5小时测量来自不同血管的血清中agKS和HA的水平。评估损伤相关变化及不同血管间差异的统计学意义。

结果

损伤后，引流注射膝关节的腘静脉中agKS水平升高最为显著，当血液到达股静脉时迅速下降。其他血管中agKS水平虽较低但相似，且在每种情况下均显著高于注射前。注射后HA水平呈现不同的变化模式。HA在引流注射膝关节的腘静脉中最高，在靠近淋巴进入循环处的头腔静脉中显著升高，在肝静脉中的水平比门静脉低50%。