Futagami K, Narazaki C, Kataoka Y, Shuto H, Oishi R
Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Chromatogr B Biomed Sci Appl. 1997 Dec 19;704(1-2):369-73. doi: 10.1016/s0378-4347(97)00476-3.
We developed a rapid and simple method for identifying 25 commonly used organophosphorus insecticides in human serum using high-performance thin-layer chromatography (HPTLC). These organophosphates were separated on plates with three different developing systems within 6-18 min and detected by means of ultraviolet radiation and coloring reactions with 4-(4-nitrobenzyl)pyridine-tetraethylenepentamine reagent (NT reagent) or palladium chloride reagent (PdCl2 reagent). Each organophosphate was accurately identified by means of the R(F) x 100 value and the spot color in three systems. The detection limits of dichlorvos, fenitrothion, malathion, methidathion, parathion and trichlorfon in serum by the liquid-liquid extraction method were 1.1, 0.12, 0.12, 0.05, 0.6 and 0.1 microg/ml, respectively. These sensitivities may be sufficient to detect those organophosphates in patient serum after acute poisoning.
我们开发了一种快速简便的方法，利用高效薄层色谱法（HPTLC）鉴定人血清中25种常用有机磷杀虫剂。这些有机磷酸盐在板上用三种不同的展开系统在6 - 18分钟内分离，并通过紫外线辐射以及与4-(4-硝基苄基)吡啶-四乙烯五胺试剂（NT试剂）或氯化钯试剂（PdCl2试剂）的显色反应进行检测。每种有机磷酸盐通过R(F)×100值和在三种系统中的斑点颜色进行准确鉴定。采用液-液萃取法时，血清中敌敌畏、杀螟硫磷、马拉硫磷、杀扑磷、对硫磷和敌百虫的检测限分别为1.1、0.12、0.12、0.05、0.6和0.1微克/毫升。这些灵敏度可能足以检测急性中毒患者血清中的那些有机磷酸盐。
