Sharma V K, Jadhav R K, Rao G J, Saraf A K, Chandra H
Medicolegal Institute, Gandhi Medical College Building, Bhopal, India.
Forensic Sci Int. 1990 Nov;48(1):21-5. doi: 10.1016/0379-0738(90)90268-4.
A method has been developed for the rapid quantitative analysis of organophosphorous and carbamate pesticides using HPLC. Good separation was obtained among the four major groups of organophosphorus pesticides [i.e. aryl phosphorothionate (methyl parathion), alkyl phosphorothionate (malathion), enolphosphate (phosphomidon, monocrotophos, dichlorvos), heterocyclic phosphorothionates (quinalphos)] and carbamates [viz. Carbaryl (Sevin) and Baygon (Dalf)] with a detection limit of 100 ng for all the pesticides. Separation was measured in terms of capacity factor (k') resolution (R) and selectivity factor (alpha ii). The method described can be used for the analysis of biological samples for the presence of organophosphorus and carbamate pesticides in the cases of poisoning. Recovery studies were made in the blood, lung and liver and found to be 85-97% with reproducibility at greater than 95%.
已开发出一种使用高效液相色谱法对有机磷和氨基甲酸酯类农药进行快速定量分析的方法。在四种主要的有机磷农药组[即硫代磷酸芳基酯（甲基对硫磷）、硫代磷酸烷基酯（马拉硫磷）、烯醇磷酸酯（磷胺、久效磷、敌敌畏）、杂环硫代磷酸酯（喹硫磷）]和氨基甲酸酯类[即西维因（甲萘威）和拜耳（残杀威）]之间实现了良好的分离，所有农药的检测限均为100纳克。分离效果通过容量因子（k'）、分辨率（R）和选择性因子（α ii）来衡量。所述方法可用于分析中毒病例中生物样品中有机磷和氨基甲酸酯类农药的存在情况。在血液、肺和肝脏中进行了回收率研究，回收率为85 - 97%，重现性大于95%。
