Amstislavskiĭ S Ia, Krivokharchenko A S, Rott N N
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
Ontogenez. 1997 Nov-Dec;28(6):412-20.
A review of our own and literature data about perspectives, problems and limitations in the use of cryoconservation techniques and methods of developmental biology for the conservation of genetic resources is presented. It is demonstrated that the use of these methods may result in the selection and possibly leads to modifications and mutations in germ cells and embryos and in this way may change the genetic structure of restored populations as compared with the original ones. The need to improve current techniques of cryoconservation and accompanying biotechnological procedures in order to abolish these undesirable genetic effects is emphasized.
本文综述了我们自己以及文献中关于利用低温保存技术和发育生物学方法来保护遗传资源的前景、问题和局限性的数据。结果表明,使用这些方法可能会导致生殖细胞和胚胎的选择,并可能引发修饰和突变,从而与原始种群相比,改变恢复种群的遗传结构。强调了改进当前低温保存技术及相关生物技术程序以消除这些不良遗传效应的必要性。