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[遗传种质库的建立及利用发育生物学方法保护珍稀动物物种。二、野生哺乳动物胚胎的获取与冷冻保存]

[The creation of genetic cryobanks and the use of the methods of developmental biology as a means for preserving rare animal species. II. The obtaining and cryopreservation of the embryos of wild mammals].

作者信息

Rott N N

出版信息

Ontogenez. 1996 Jul-Aug;27(4):245-55.

PMID:8975202
Abstract

A review of published data on obtaining, cryoconservation, and subsequent development of the embryos of wild animals. Development of the embryos until birth after freezing-thawing and transplantation of the embryos to recipient females was followed in eight species. Studies are mostly carried out in the zoos and some universities of USA, Canada, and UK. The Red Data Book species and artificially bred species are the main objects. In these studies, diverse methods of developmental biology were used: induced oocyte maturation, artificial fertilization and cultivation of the embryos in vitro, transplantation of the embryos to foster mothers of the same or closely related species, and obtaining of chimeras.

摘要

对已发表的关于野生动物胚胎获取、冷冻保存及后续发育数据的综述。在八个物种中追踪了胚胎经冻融及移植到受体雌性动物后直至出生的发育情况。研究大多在美国、加拿大和英国的动物园及一些大学开展。主要研究对象是《濒危物种红色名录》中的物种及人工繁育物种。在这些研究中,采用了多种发育生物学方法:诱导卵母细胞成熟、人工授精及体外培养胚胎、将胚胎移植到同种或近缘物种的代孕母体以及获得嵌合体。

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