Greinert R, Staerk H, Stier A, Weller A
J Biochem Biophys Methods. 1979 May;1(2):77-83. doi: 10.1016/0165-022x(79)90014-9.
E (eosin)-type delayed fluorescence depolarization studies extend the time range for the measurement of rotational diffusion to microseconds and ms, thereby allowing investigation of slow rotational movement of macromolecules like membrane proteins. An apparatus is described for the determination of time-dependent anisotropy in this interesting time range. The method has been tested on eosin-labelled cytochrome P-450 incorporated into phospholipid membrane vesicles.
