用于测量膜蛋白旋转扩散的荧光三重态探针。
Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins.
作者信息
Johnson P, Garland P B
出版信息
Biochem J. 1982 Apr 1;203(1):313-21. doi: 10.1042/bj2030313.
Abstract
We have previously described a method for measuring the rotational diffusion of membrane proteins by using fluorescent triplet probes [Johnson & Garland (1981) FEBS Lett. 135, 252-256]. We now describe the criteria by which the suitability of such probes may be judged. In general, the greatest sensitivity is achievable with probes where the ratio of the quantum yields for prompt fluorescene (phi f) and triplet formation (phi t) are high, as with Rhodamine (phi f/phi t congruent to 10(3)). However, considerations of heat generation at the sample membrane, of time resolution of fast rotations and of irreversible bleaching of the fluorescent probe also apply. The immediate environment of a probe molecule at a membrane protein must also be important in determining the performance of a given probe. Nevertheless, we describe guidelines for evaluating the likely usefulness of fluorescent triplet probes in measurements of membrane protein rotation.
摘要
我们之前描述了一种通过使用荧光三重态探针来测量膜蛋白旋转扩散的方法[约翰逊和加兰（1981年），《欧洲生物化学学会联合会快报》135卷，252 - 256页]。我们现在描述判断此类探针适用性的标准。一般来说，对于那些即时荧光（φf）和三重态形成（φt）的量子产率之比很高的探针，能实现最大灵敏度，比如罗丹明（φf/φt约为10³）。然而，样品膜处的发热、快速旋转的时间分辨率以及荧光探针的不可逆漂白等因素也需要考虑。探针分子在膜蛋白处的直接环境对于确定给定探针的性能也必定很重要。尽管如此，我们还是描述了评估荧光三重态探针在膜蛋白旋转测量中可能有用性的指导原则。
相似文献
1
Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins.用于测量膜蛋白旋转扩散的荧光三重态探针。
Biochem J. 1982 Apr 1;203(1):313-21. doi: 10.1042/bj2030313.
2
Carbocyanine dyes used as fluorescent triplet probes for measuring slow rotational diffusion of lipids in membranes.
FEBS Lett. 1983 Mar 21;153(2):391-4. doi: 10.1016/0014-5793(83)80650-4.
3
Rotational and translational diffusion of size-dependent fluorescent probes in homogeneous and heterogeneous environments.尺寸依赖型荧光探针在均相和非均相环境中的旋转和平移扩散。
Phys Chem Chem Phys. 2018 Oct 7;20(37):24045-24057. doi: 10.1039/c8cp03873b. Epub 2018 Sep 11.
4
Phosphorescence of protein-bound eosin and erythrosin. A possible probe for measurements of slow rotational mobility.蛋白质结合的曙红和赤藓红的磷光。一种测量缓慢旋转迁移率的可能探针。
Biochem J. 1979 Dec 1;183(3):561-72. doi: 10.1042/bj1830561.
5
A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 1: Preparation and properties of a triplet probe.一种用于测量大分子缓慢旋转扩散的光谱技术。1：三重态探针的制备与性质
Biochemistry. 1976 Aug 24;15(17):3653-6. doi: 10.1021/bi00662a001.
6
Submicrosecond phospholipid dynamics using a long-lived fluorescence emission anisotropy probe.使用长寿命荧光发射各向异性探针的亚微秒级磷脂动力学
Biophys J. 1996 Oct;71(4):1837-52. doi: 10.1016/S0006-3495(96)79384-0.
7
Depolarization of fluorescence depletion. A microscopic method for measuring rotational diffusion of membrane proteins on the surface of a single cell.荧光耗尽去极化。一种用于测量单个细胞表面膜蛋白旋转扩散的显微方法。
FEBS Lett. 1981 Sep 28;132(2):252-6. doi: 10.1016/0014-5793(81)81172-6.
8
Laser-driven microsecond temperature cycles analyzed by fluorescence polarization microscopy.通过荧光偏振显微镜分析的激光驱动微秒级温度循环。
Biophys J. 2006 Apr 15;90(8):2958-69. doi: 10.1529/biophysj.105.075168. Epub 2006 Jan 27.
9
Protein rotational dynamics investigated with a dual EPR/optical molecular probe. Spin-labeled eosin.用双EPR/光学分子探针研究蛋白质旋转动力学。自旋标记的曙红。
Biophys J. 1993 Mar;64(3):605-13. doi: 10.1016/S0006-3495(93)81419-X.
引用本文的文献
1
Photophysical studies of bioconjugated ruthenium metal-ligand complexes incorporated in phospholipid membrane bilayers.磷脂膜双层中结合的钌金属配体配合物的光物理研究。
Inorg Chem. 2013 Oct 7;52(19):10835-45. doi: 10.1021/ic400706u. Epub 2013 Sep 24.
2
Increased protein glycation in diabetes mellitus is associated with decreased aspirin-mediated protein acetylation and reduced sensitivity of blood platelets to aspirin.糖尿病中蛋白质糖基化增加与阿司匹林介导的蛋白质乙酰化减少以及血小板对阿司匹林的敏感性降低有关。
J Mol Med (Berl). 2005 Feb;83(2):148-58. doi: 10.1007/s00109-004-0600-x. Epub 2004 Nov 10.
3
Polarized fluorescence depletion reports orientation distribution and rotational dynamics of muscle cross-bridges.偏振荧光耗尽法可报告肌肉横桥的取向分布和旋转动力学。
Biophys J. 2002 Aug;83(2):1050-73. doi: 10.1016/S0006-3495(02)75230-2.
4
Cytoplasmic viscosity near the cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery.细胞膜附近的细胞质黏度：通过全内反射荧光漂白恢复技术测量小荧光溶质的平移扩散
Biophys J. 1996 Aug;71(2):1140-51. doi: 10.1016/S0006-3495(96)79316-5.
5
Fluorescence lifetime imaging microscopy (flimscopy). Methodology development and application to studies of endosome fusion in single cells.荧光寿命成像显微镜（FLIMscopy）。方法学的发展及其在单细胞内吞体融合研究中的应用。
Biophys J. 1993 Mar;64(3):676-85. doi: 10.1016/S0006-3495(93)81427-9.
6
Segmental motion and rotational diffusion of the Ca2+-translocating adenosine triphosphatase of sarcoplasmic reticulum, measured by time-resolved phosphorescence depolarization.通过时间分辨磷光去极化测量肌质网Ca2+转运三磷酸腺苷酶的节段运动和旋转扩散。
Biochem J. 1983 Jul 1;213(1):67-74. doi: 10.1042/bj2130067.
7
The lateral diffusion of lipid probes in the surface membrane of Schistosoma mansoni.曼氏血吸虫表面膜中脂质探针的侧向扩散。
J Cell Biol. 1986 Sep;103(3):807-18. doi: 10.1083/jcb.103.3.807.
8
Fluorescent microtubules break up under illumination.荧光微管在光照下会分解。
J Cell Biol. 1988 Sep;107(3):1011-24. doi: 10.1083/jcb.107.3.1011.
9
Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes.用于测量溶液和膜中旋转扩散的偏振荧光光漂白恢复技术。
Biophys J. 1988 Apr;53(4):575-91. doi: 10.1016/S0006-3495(88)83137-0.
10
Analysis of rhodamine and fluorescein-labeled F-actin diffusion in vitro by fluorescence photobleaching recovery.通过荧光漂白恢复技术对体外罗丹明和荧光素标记的F-肌动蛋白扩散进行分析。
Biophys J. 1988 Nov;54(5):801-15. doi: 10.1016/S0006-3495(88)83018-2.
本文引用的文献
1
2
Fluorescence photobleaching recovery: control of laser intensities with an acousto-optic modulator.荧光光漂白恢复：用声光调制器控制激光强度。
Biophys J. 1981 Mar;33(3):481-2. doi: 10.1016/S0006-3495(81)84910-7.
3
Depolarization of fluorescence depletion. A microscopic method for measuring rotational diffusion of membrane proteins on the surface of a single cell.荧光耗尽去极化。一种用于测量单个细胞表面膜蛋白旋转扩散的显微方法。
FEBS Lett. 1981 Sep 28;132(2):252-6. doi: 10.1016/0014-5793(81)81172-6.
4
Optical excitation of the eosin-human serum albumin complex.曙红-人血清白蛋白复合物的光激发
Photochem Photobiol. 1967 Oct;6(10):721-31. doi: 10.1111/j.1751-1097.1967.tb08737.x.
5
The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light.一种用于落射光荧光显微镜的、带有可互换二向色镜的垂直照明器的使用。
Z Wiss Mikrosk. 1967 Nov;68(3):129-42.
6
Spectroscopic technique for studying protein rotation in membranes.用于研究膜中蛋白质旋转的光谱技术。
Nat New Biol. 1973 Oct 24;245(147):249-51. doi: 10.1038/newbio245249a0.
7
A microfluorimetric study of translational diffusion in erythrocyte membranes.红细胞膜中平移扩散的微量荧光测定研究。
Biochim Biophys Acta. 1974 Nov 15;367(3):282-94. doi: 10.1016/0005-2736(74)90085-6.
8
Measurement of membrane protein lateral diffusion in single cells.单细胞中膜蛋白侧向扩散的测量。
Science. 1976 Feb 6;191(4226):466-8. doi: 10.1126/science.1246629.
9
Characterization of nucleotide binding sites on chloroplast coupling factor 1.叶绿体偶联因子1上核苷酸结合位点的特性分析
Biochemistry. 1975 Jul;14(13):2968-75. doi: 10.1021/bi00684a027.
10
A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 2: Determination of rotational correlation times of proteins in solution.
Biochemistry. 1976 Aug 24;15(17):3657-61. doi: 10.1021/bi00662a002.
Zotero 插件