Tanaka H, Nagai H, Maeda Y
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Life Sci. 1998;62(13):PL169-74. doi: 10.1016/s0024-3205(98)00047-2.
In order to study the role of IL-4 and IL-5 in allergen-induced airway hyperresponsiveness in mice, the effect of the combined administration of anti-IL-4 and anti-IL-5 monoclonal antibodies (mAbs) on IgE response, airway inflammation and airway hyperresponsiveness was studied in sensitized Balb/c mice. Three inhalations of antigen caused an increase in the number of eosinophils in bronchoalveolar lavage fluid and in airway responsiveness to acetylcholine, with a significant elevation in the serum antigen-specific IgE level. Anti-IL-4 mAb inhibited IgE production but did not affect airway eosinophilia or hyperresponsiveness. Moreover, anti-IL-5 mAb inhibited airway eosinophilia but did not affect IgE production or airway hyperresponsiveness. The combined administration of anti-IL4 and anti-IL-5 mAbs, however, inhibited IgE antibody production, airway eosinophilia and hyperresponsiveness. These results suggest that inhibitory action of IL-4 and IL-5 in combination can effectively suppress the onset of antigen-induced airway hyperresponsiveness in mice.
为了研究白细胞介素-4（IL-4）和白细胞介素-5（IL-5）在变应原诱导的小鼠气道高反应性中的作用，在致敏的Balb/c小鼠中研究了联合给予抗IL-4和抗IL-5单克隆抗体（mAb）对IgE反应、气道炎症和气道高反应性的影响。三次吸入抗原导致支气管肺泡灌洗液中嗜酸性粒细胞数量增加以及气道对乙酰胆碱的反应性增加，血清抗原特异性IgE水平显著升高。抗IL-4 mAb抑制IgE产生，但不影响气道嗜酸性粒细胞增多或高反应性。此外，抗IL-5 mAb抑制气道嗜酸性粒细胞增多，但不影响IgE产生或气道高反应性。然而，联合给予抗IL-4和抗IL-5 mAb可抑制IgE抗体产生、气道嗜酸性粒细胞增多和高反应性。这些结果表明，IL-4和IL-5的联合抑制作用可有效抑制小鼠抗原诱导的气道高反应性的发生。
