Morita Y, Moriai T, Takiyama Y, Makino I
Second Department of Internal Medicine, Asahikawa Medical College, Japan.
Int J Pancreatol. 1998 Feb;23(1):41-50. doi: 10.1007/BF02787502.
This new animal cell line may be a useful model to study the effect of growth factors on malignant cell proliferation and differentiation in both in vivo and in vitro systems.
We established a new pancreatic cancer cell line from pancreatic cancer in the hamster (HPC) induced by N-nitrosobis(2-oxopropyl)amine (BOP) and characterized its morphological, pathological, and biological patterns.
Cells grew rapidly, with a doubling time of 22.5 h. Chromosome number ranged from 33 to 144, and flow cytometric analysis showed two peaks of DNA distribution as a proliferative pattern. Ultrastructural analyses using transmission and scanning electron microscopy of HPC cells revealed desmosomes and loose interdigitation, with pseudopods and microvilli on the cell surface. The overexpression of epidermal growth factor (EGF) receptors on HPC cells was shown by immunohistochemistry. Binding characteristics and biological activity of EGF and type alpha transforming growth factor (TGF-alpha) were studied. TGF-alpha stimulated DNA synthesis in a dose-dependent manner, whereas EGF was without effect. Scatchard analysis of 125I-EGF binding data at pH 7.4 indicated the presence of two orders of binding sites, where that of 125I-TGF-alpha showed only a single order. Regarding the effect of pH on 125I-EGF or 125I-TGF-alpha dissociation, one-half maximal dissociation of 125I-EGF or 125I-TGF-alpha occurred at pH 6.0 or 6.5, respectively. Characteristics of the EGF receptor are similar to those of cultured human pancreatic cancer cells.
这种新的动物细胞系可能是一种有用的模型，可用于研究生长因子在体内和体外系统中对恶性细胞增殖和分化的影响。
我们从N-亚硝基双（2-氧代丙基）胺（BOP）诱导的仓鼠胰腺癌中建立了一种新的胰腺癌细胞系（HPC），并对其形态、病理和生物学模式进行了表征。
细胞生长迅速，倍增时间为22.5小时。染色体数范围为33至144，流式细胞术分析显示DNA分布有两个峰，呈增殖模式。使用透射和扫描电子显微镜对HPC细胞进行的超微结构分析显示有桥粒和松散的指状交叉，细胞表面有伪足和微绒毛。免疫组织化学显示HPC细胞上表皮生长因子（EGF）受体过表达。研究了EGF和α型转化生长因子（TGF-α）的结合特性和生物学活性。TGF-α以剂量依赖性方式刺激DNA合成，而EGF无作用。在pH 7.4下对125I-EGF结合数据进行Scatchard分析表明存在两个结合位点级别，而125I-TGF-α仅显示一个级别。关于pH对125I-EGF或125I-TGF-α解离的影响，125I-EGF或125I-TGF-α的半数最大解离分别发生在pH 6.0或6.5。EGF受体的特性与培养的人胰腺癌细胞相似。
