Hamada J, Takeichi N, Kobayashi H
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Cancer Res. 1988 Sep 15;48(18):5129-32.
Three highly metastatic clones and two weakly metastatic clones were obtained from a spontaneously arising mammary carcinoma in an SHR rat. The difference in their capacity to generate metastatic ability was recognized when the tumor cells were implanted s.c. but not when they were implanted i.v. This evidence possibly indicates that the difference in the metastatic capacity of these clones is caused by different potential for detachment from the primary site and for intravasation during the various steps of metastasis. There are no differences between highly and weakly metastatic clones with regard to their in vitro growth characteristics (doubling time, saturation density, plating efficiency, etc.), homotypic aggregation, and adhesiveness to plastic matrices and fibroblast monolayers. Therefore, we used a dye transfer method to examine the relationship between the metastatic capacity of tumor cells and the capacity of tumor cells to make junctional communication with normal fibroblasts. We found that the incidence of intercellular communication between weakly metastatic clone cells and fibroblasts (derived from normal s.c. tissues and tumor tissues) was significantly higher than that between highly metastatic clone cells and fibroblasts. These results suggest that junctional communication between tumor cells and normal fibroblasts may play a part in the early stage of cancer metastasis.
从一只自发性乳腺肿瘤的SHR大鼠中获得了三个高转移克隆和两个低转移克隆。当肿瘤细胞皮下接种时，它们产生转移能力的差异得到了认可，但静脉注射时则没有这种差异。这一证据可能表明，这些克隆转移能力的差异是由转移各个步骤中从原发部位脱离和进入血管的不同潜力所导致的。高转移克隆和低转移克隆在体外生长特性（倍增时间、饱和密度、接种效率等）、同型聚集以及对塑料基质和成纤维细胞单层的粘附性方面没有差异。因此，我们使用染料转移方法来研究肿瘤细胞的转移能力与肿瘤细胞与正常成纤维细胞进行连接通讯的能力之间的关系。我们发现，低转移克隆细胞与成纤维细胞（源自正常皮下组织和肿瘤组织）之间的细胞间通讯发生率显著高于高转移克隆细胞与成纤维细胞之间的通讯发生率。这些结果表明，肿瘤细胞与正常成纤维细胞之间的连接通讯可能在癌症转移的早期阶段起作用。
