Gasperini S, Zambello R, Agostini C, Trentin L, Tassinari C, Cadrobbi P, Semenzato G, Cassatella M A
Department of General Pathology, University of Verona, Italy.
AIDS. 1998 Mar 5;12(4):373-9. doi: 10.1097/00002030-199804000-00005.
To determine the ability of neutrophils isolated from HIV-seropositive patients to produce proinflammatory cytokines.
The in vitro responsiveness of polymorphonuclear neutrophils (PMN) and peripheral blood mononuclear cells (PBMC) to lipopolysaccharide (LPS), used in the presence or absence of interferon (IFN)gamma, was determined in 47 HIV-positive patients with advanced stages of virus infection.
Cytokines in cell-free supernatants were measured by enzyme-linked immunosorbent assay or radioimmunoassay.
Cell-free supernatants from PMN isolated from the peripheral blood of HIV-positive patients and stimulated with LPS contained increased amounts of tumour necrosis factor (TNF)-alpha and interleukin (IL)-8 with respect to supernatants obtained from PMN of normal donors. In contrast, release of IL-1beta and IL-1ra (IL-1 receptor antagonist) in response to LPS, or LPS plus IFNgamma, was found to be lower in PMN from HIV-positive patients than in PMN from controls, but was significant only in the case of IL-1ra. Furthermore, the release of IL-12 induced by LPS or LPS plus IFNgamma did not significantly differ between PMN from HIV-positive patients and healthy donors. Concerning PBMC, the production of TNF-alpha and IL-12 in response to LPS, or LPS plus IFNgamma, was found to be significantly higher in cells isolated from HIV-positive patients, whereas the release of IL-1beta was significantly lower. In the case of IL-8, no statistically significant difference was found between PBMC isolated from HIV-positive patients and healthy donors.
Collectively, the data suggest that in HIV-positive patients with advanced stages of disease, the ability of PMN to produce specific cytokines in response to LPS is significantly altered. Alterations in this ability might contribute to the evolution of HIV disease.
确定从HIV血清阳性患者中分离出的中性粒细胞产生促炎细胞因子的能力。
在47例处于病毒感染晚期的HIV阳性患者中，测定了多形核中性粒细胞（PMN）和外周血单核细胞（PBMC）在有或无干扰素（IFN）γ存在的情况下对脂多糖（LPS）的体外反应性。
通过酶联免疫吸附测定法或放射免疫测定法测量无细胞上清液中的细胞因子。
与从正常供体的PMN获得的上清液相比，从HIV阳性患者外周血中分离并经LPS刺激的PMN的无细胞上清液中，肿瘤坏死因子（TNF）-α和白细胞介素（IL）-8的含量增加。相反，发现HIV阳性患者的PMN对LPS或LPS加IFNγ的反应中，IL-1β和IL-1ra（IL-1受体拮抗剂）的释放低于对照组的PMN，但仅在IL-1ra的情况下具有统计学意义。此外，LPS或LPS加IFNγ诱导的IL-12释放在HIV阳性患者的PMN和健康供体之间没有显著差异。关于PBMC，发现从HIV阳性患者分离的细胞对LPS或LPS加IFNγ的反应中，TNF-α和IL-12的产生显著更高，而IL-1β的释放显著更低。对于IL-8，在从HIV阳性患者和健康供体分离的PBMC之间未发现统计学上的显著差异。
总体而言，数据表明在疾病晚期的HIV阳性患者中，PMN对LPS产生特异性细胞因子的能力发生了显著改变。这种能力的改变可能有助于HIV疾病的进展。
