Tani K, Su S B, Utsunomiya I, Oppenheim J J, Wang J M
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
Eur J Immunol. 1998 Feb;28(2):502-7. doi: 10.1002/(SICI)1521-4141(199802)28:02<502::AID-IMMU502>3.0.CO;2-5.
The neutrophil chemotactic cytokine, IL-8, has been reported to also chemoattract T lymphocytes in vitro and in vivo. Previously we showed that freshly isolated T cells migrated in response to IL-8, but incubation of T cells at 37 degrees C resulted in progressively decreased levels of IL-8 binding sites on T cells in association with reduced chemotactic responses. However, this reduced binding and migration of cultured T cells in response to IL-8 can be prevented by the presence of mononuclear cells in the culture. In order to define the factor(s) responsible for the restoration of T cell binding and migration in response to IL-8, we examined the effects of various cytokines. Addition of IFN-gamma in cultured T cells maintained both the CXC chemokine receptor CXCR1 and CXCR2 binding sites for IL-8 on these cells to the level comparable to that expressed on freshly purified T cells accompanied by an almost complete restoration of their chemotactic response to IL-8. The results suggest that Th1 cytokine, IFN-gamma, produced by mononuclear cells stimulated by proinflammatory signals may play an important role in regulating IL-8 receptor expression on T cells and in sustaining the function of these cells in response to IL-8.
据报道，中性粒细胞趋化细胞因子白细胞介素8（IL-8）在体外和体内也能趋化T淋巴细胞。此前我们发现，新鲜分离的T细胞会对IL-8产生迁移反应，但将T细胞在37℃孵育会导致T细胞上IL-8结合位点水平逐渐降低，同时趋化反应也减弱。然而，培养的T细胞中存在单核细胞时，可防止其对IL-8的结合和迁移能力降低。为了确定负责恢复T细胞对IL-8的结合和迁移能力的因子，我们检测了多种细胞因子的作用。在培养的T细胞中添加干扰素-γ（IFN-γ），可使这些细胞上IL-8的CXC趋化因子受体CXCR1和CXCR2结合位点维持在与新鲜纯化T细胞上表达水平相当的程度，同时其对IL-8的趋化反应几乎完全恢复。结果表明，由促炎信号刺激的单核细胞产生的Th1细胞因子IFN-γ，可能在调节T细胞上IL-8受体表达以及维持这些细胞对IL-8的反应功能方面发挥重要作用。
