Schramm W, Wade S E, Barriga Angulo G, Castillo Torres P, Burgess-Cassler A
Saliva Diagnostic Systems, Inc., Vancouver, Washington 98682-2444, USA.
Clin Diagn Lab Immunol. 1998 Mar;5(2):263-5. doi: 10.1128/CDLI.5.2.263-265.1998.
We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).
我们开发了一种免疫层析全血检测法（WBT），可从指尖采血中检测出针对1型和2型人类免疫缺陷病毒（HIV-1和HIV-2）的抗体。以血清或血浆酶免疫测定法（EIA）作为参考，WBT的灵敏度和特异性分别为99.41%（1018例确诊阳性患者）和99.89%（941例未感染患者）。使用18份HIV-2血清、23个HIV-1血清转化样本组和一个低滴度性能样本组（代替全血），WBT的性能与已获许可的EIA和免疫印迹法相当。
