Austin L M, Coven T R, Bhardwaj N, Steinman R, Krueger J G
Laboratory of Investigative Dermatology, The Rockefeller University, New York, New York, USA.
J Cutan Pathol. 1998 Feb;25(2):79-88. doi: 10.1111/j.1600-0560.1998.tb01694.x.
The onset and persistence of psoriatic lesions are linked to the presence of an inflammatory infiltrate of CD3+ lymphocytes that includes CD4+ and CD8+ subsets. Since a primary susceptibility factor for psoriasis is the Class I HLA-Cw6 molecule, we set out to learn more about the features of the epidermal CD8+ lymphocytes. The markers tested were GMP-17, a cytotoxic granule protein found in activated cytotoxic lymphocytes (CTLs), and the alpha chain of the IL-2 receptor (CD25), a plasma membrane molecule found on activated T cells. Lymphocytes in lesional skin expressed the GMP-17 protein, whereas lymphocytes in non-lesional skin, resolving lesional skin and normal skin had little or no GMP-17. By flow cytometry analysis, lesional epidermal GMP-17+ cells were CD8+CD3+, with a subpopulation expressing the activation marker CD25+. Due to the abundance of activated GMP-17+CD8+CD3+ lymphocytes (the phenotype of activated cytotoxic cells) in psoriatic lesions compared to non-lesional and normal skin, we hypothesize that they are contributing directly to the psoriatic phenotype.
银屑病皮损的发生和持续存在与包括CD4+和CD8+亚群的CD3+淋巴细胞炎性浸润的存在有关。由于银屑病的一个主要易感因素是I类HLA - Cw6分子，我们着手进一步了解表皮CD8+淋巴细胞的特征。检测的标志物为GMP - 17，一种在活化的细胞毒性淋巴细胞（CTLs）中发现的细胞毒性颗粒蛋白，以及IL - 2受体的α链（CD25），一种在活化T细胞上发现的质膜分子。皮损皮肤中的淋巴细胞表达GMP - 17蛋白，而非皮损皮肤、消退期皮损皮肤和正常皮肤中的淋巴细胞几乎不表达或不表达GMP - 17。通过流式细胞术分析，皮损表皮GMP - 17+细胞为CD8+CD3+，其中一个亚群表达活化标志物CD25+。与非皮损和正常皮肤相比，银屑病皮损中存在大量活化的GMP - 17+CD8+CD3+淋巴细胞（活化细胞毒性细胞的表型），因此我们推测它们直接促成了银屑病表型。
