无辅脂酶活性的猪胰脂肪酶的制备。

Preparation of porcine pancreatic lipase free of co-lipase activity.

作者信息

Donnér J

出版信息

Acta Chem Scand B. 1976;30(5):430-4. doi: 10.3891/acta.chem.scand.30b-0430.

DOI:10.3891/acta.chem.scand.30b-0430

Abstract

Porcine pancreatic lipase LB was purified and released from co-lipase activity by reduction with beta-mercaptoethanol in the presence of guanidine chloride. The amino acid and carbohydrate composition of the enzyme is presented. The following physical constants were measured or calculated: Molecular weight 52 000, sedimentation coefficient (s degrees 20, w) 4.0 S, diffusion coefficient (D degrees 20, w) 6.7 X 10(-7) cm2 s-1. Stokes' radius (r) 30.3 A, partial specific volume (v) 0.72 cm3 g-1, frictional ratio (f/f0) 1.23 and isoelectric point (pI) 5.18.

摘要

猪胰脂肪酶LB在氯化胍存在下用β-巯基乙醇还原，从而从共脂肪酶活性中纯化并释放出来。文中给出了该酶的氨基酸和碳水化合物组成。测定或计算了以下物理常数：分子量52000，沉降系数（s₂₀,w°）4.0 S，扩散系数（D₂₀,w°）6.7×10⁻⁷ cm² s⁻¹，斯托克斯半径（r）30.3 Å，比容（v）0.72 cm³ g⁻¹，摩擦比（f/f₀）1.23，等电点（pI）5.18。