Sanchez Navarro M R, Wangensteen Fuentes R, Oliver Almendros C
Departamento de Bioquímica, Hospital Universitario San Cecilio, Granada.
An Med Interna. 1998 Jan;15(1):13-7.
We studied lactate-dehydrogenase (LD) isoenzymes and creatinekinase MM (CKMM) isoforms MM1, MM2 and MM3, in the serum of 18 patients with nontraumatic acute rhabdomyolysis, to test the utility of these markers in the diagnosis and disease evolution. The isoenzymes were separated by electrophoresis on agarose gel and were quantified with a densitometer. We studied the correlation between CKMM isoforms calculating the MM3/MM1 ratio, establishing the reference values from control group of 36 healthy adults. MM3 and MM3/MM1 ratio values were significantly increased in patients with rhabdomyolysis (p < 0.001) and progressively decreased coinciding with signs of getting better, showing 10 days after similar values of control group. LD4 and LD5 isoenzymes were significantly increased (p < 0.001) keeping elevated until 8-10 days when they showed a significant decrease compared with admission values (p < 0.05) but keeping elevated respect to control (p < 0.01).
我们研究了18例非创伤性急性横纹肌溶解症患者血清中的乳酸脱氢酶（LD）同工酶以及肌酸激酶MM（CKMM）亚型MM1、MM2和MM3，以检验这些标志物在诊断和疾病进展中的效用。通过琼脂糖凝胶电泳分离同工酶，并用密度计进行定量。我们通过计算MM3/MM1比值研究了CKMM亚型之间的相关性，从36名健康成年人的对照组中确定参考值。横纹肌溶解症患者的MM3和MM3/MM1比值显著升高（p < 0.001），并随着病情好转的迹象逐渐下降，在10天后显示出与对照组相似的值。LD4和LD5同工酶显著升高（p < 0.001），直至8 - 10天，与入院时的值相比显著下降（p < 0.05），但相对于对照组仍保持升高（p < 0.01）。
