Nieves M A, Vahle J, Ackermann M, Howard M, Dietz A B, Carpenter S L, Cheville N
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Am J Vet Res. 1998 Mar;59(3):359-62.
To produce and characterize cell lines from canine primary appendicular osteosarcomas that induce transplantable tumors in athymic nude mice.
57 six- to 8-week-old female athymic nude mice.
Canine primary appendicular osteosarcoma tumors were harvested and cell lines were produced. Canine osteosarcoma (COSCA)-Toby (COSCA-T; 10 mice), COSCA-Princess (COSCA-Pr; 16) or canine osteosarcoma D-17 (ATCC CCL-183; 31) cells were injected into the proximal portion of the left tibia of nude mice to evaluate tumor production from each cell line; the right tibia served as the control. Tibial measurements were taken on alternating days to evaluate tumor growth during a 6-month period. Student's t-tests were used to determine whether size of the proximal portion of the left and right tibias differed significantly during the observation period.
88% of mice receiving COSCA-Pr and 50% of mice receiving COSCA-T cells developed a tumor at the injection site by 9 days after implantation. The D-17 cells induced tumors in 50% of injected tibias; however, tumors were not detected for 79 days. Tumors generated from COSCA-Pr and COSCA-T cells in nude mice were histologically similar to the canine tumor from which they were developed.
New osteosarcoma cell lines that can reliably and rapidly induce transplantable tumors in nude mice were developed.
Use of cell lines will allow evaluation of new treatments of canine primary appendicular osteosarcoma in a nude mouse model.
从犬原发性四肢骨肉瘤中培养细胞系并进行特性鉴定，该细胞系可在无胸腺裸鼠中诱发可移植性肿瘤。
57只6至8周龄的雌性无胸腺裸鼠。
采集犬原发性四肢骨肉瘤肿瘤并培养细胞系。将犬骨肉瘤（COSCA）-托比（COSCA-T；10只小鼠）、COSCA-普琳西丝（COSCA-Pr；16只）或犬骨肉瘤D-17（ATCC CCL-183；31只）细胞注射到裸鼠左胫骨近端，以评估各细胞系产生肿瘤的情况；右胫骨作为对照。每隔一天测量胫骨，以评估6个月期间肿瘤的生长情况。采用学生t检验来确定观察期内左、右胫骨近端大小是否存在显著差异。
接受COSCA-Pr的小鼠中有88%以及接受COSCA-T细胞的小鼠中有50%在植入后9天内在注射部位出现肿瘤。D-17细胞在50%的注射胫骨中诱发肿瘤；然而，79天内未检测到肿瘤。裸鼠中由COSCA-Pr和COSCA-T细胞产生的肿瘤在组织学上与它们所源自的犬肿瘤相似。
开发出了能够在裸鼠中可靠且快速诱发可移植性肿瘤的新型骨肉瘤细胞系。
使用细胞系将能够在裸鼠模型中评估犬原发性四肢骨肉瘤的新治疗方法。
