Maldonado M C, Strasser de Saad A M
Departmento de Microbiología Industrial y General, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Argentina.
J Ind Microbiol Biotechnol. 1998 Jan;20(1):34-8. doi: 10.1038/sj.jim.2900470.
Production of pectinesterase and polygalacturonase by Aspergillus niger was studied in submerged and solid-state fermentation systems. With pectin as a sole carbon source, pectinesterase and polygalacturonase production were four and six times higher respectively in a solid state system than in a submerged fermentation system and required a shorter time for enzyme production. The addition of glucose increased pectinesterase and polygalacturonase production in the solid state system but in submerged fermentation the production was markedly inhibited. A comparison of enzyme productivities showed that those determined for pectinesterase and polygalacturonase with pectin as a carbon source were three and five times higher by using the solid state rather than the submerged fermentation system. The productivities of the two enzymes were affected by glucose in both fermentation systems. The membranes of cells from the solid state fermentation showed increased levels of C18:1, C16:0 and C18:0 fatty acids. Differences in the regulation of enzyme synthesis by Aspergillus niger depended on the fermentation system, favoring the solid state over the submerged fermentation for pectinase production.
研究了黑曲霉在深层发酵和固态发酵系统中果胶酯酶和多聚半乳糖醛酸酶的产生情况。以果胶作为唯一碳源时，固态系统中果胶酯酶和多聚半乳糖醛酸酶的产量分别比深层发酵系统高4倍和6倍，且酶产生所需时间更短。添加葡萄糖可提高固态系统中果胶酯酶和多聚半乳糖醛酸酶的产量，但在深层发酵中产量受到显著抑制。酶生产率的比较表明，以果胶作为碳源时，固态发酵系统中果胶酯酶和多聚半乳糖醛酸酶的生产率比深层发酵系统分别高3倍和5倍。在两种发酵系统中，这两种酶的生产率均受葡萄糖影响。固态发酵细胞的细胞膜中C18:1、C16:0和C18:0脂肪酸水平升高。黑曲霉对酶合成的调控差异取决于发酵系统，在果胶酶生产方面，固态发酵优于深层发酵。
