Fiedurek J, Szczodrak J, Rogalski J
Department of Applied Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
J Appl Bacteriol. 1995 Apr;78(4):409-12. doi: 10.1111/j.1365-2672.1995.tb03426.x.
A simple method for the immobilization of Aspergillus niger mycelium producing polygalacturonase (PG) and pectinesterase (PE) is described. Fungal conidia were immobilized on wheat, rye, barley, peas, buckwheat and mustards seeds. Spongy mycelia overgrowing the seed surfaces on mineral medium with pectin produced extracellular PG and PE; the highest production was reached on the wheat carrier. Some of the variables influencing the enzymatic activity have been optimized. After every 24 h, a culture liquid with 6.8-7.8 U of PG ml-1 and 7.0-10.1 U of PE ml-1 was obtained. This procedure also made possible repeated batch enzyme production and, as many as eight subsequent 24-h batches could be fermented by using the same carrier without any loss of PG activity. The addition of sodium orthovanadate (1 mmol) into the medium with pectin caused a significant increase in PG and PE activity produced by free cells of A. niger (by 1.59-fold and 1.67-fold respectively), and only 0.47-fold of PG activity in case of the immobilized mycelium.
描述了一种固定化产多聚半乳糖醛酸酶（PG）和果胶酯酶（PE）的黑曲霉菌丝体的简单方法。将真菌分生孢子固定在小麦、黑麦、大麦、豌豆、荞麦和芥菜种子上。在含有果胶的矿物培养基上，种子表面生长出的海绵状菌丝体产生细胞外PG和PE；在小麦载体上产量最高。对一些影响酶活性的变量进行了优化。每24小时可获得含有6.8 - 7.8 U/ml PG和7.0 - 10.1 U/ml PE的培养液。该方法还实现了酶的重复分批生产，并且使用相同载体可连续发酵多达八批24小时的批次，而PG活性没有任何损失。向含有果胶的培养基中添加原钒酸钠（1 mmol）会使黑曲霉游离细胞产生的PG和PE活性显著增加（分别增加1.59倍和1.67倍），而对于固定化菌丝体，PG活性仅增加0.47倍。
