Benoit De Coignac A, Bisbal C, Lebleu B, Salehzada T
IGMM-CNRS-UMR 5535, 1919 route de Mende, 34293, Montpellier Cedex 5, France.
Gene. 1998 Mar 16;209(1-2):149-56. doi: 10.1016/s0378-1119(98)00040-7.
The 2-5A/RNase L system is one of the pathways induced by interferon (IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor (RLI). Human RLI (Hu-RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI (Mu-RLI). Hu-RLI and Mu-RLI protein have 98% amino acid identity. Mu-RLI is functionally homologous to Hu-RLI, and all the structural features and amino acid sequence motifs of Hu-RLI are conserved in Mu-RLI. Moreover, reticulocyte lysate translated Mu-RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu-RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation.
2-5A/RNase L系统是干扰素（IFN）诱导的信号通路之一。它在IFN的抗病毒和抗增殖活性中起主要作用。最近，我们发现RNase L的活性可被一种蛋白质抑制剂——RNase L抑制剂（RLI）所抑制。人RLI（Hu-RLI）已被克隆并鉴定。在此，我们描述了编码小鼠RLI（Mu-RLI）的cDNA的分离和鉴定。Hu-RLI和Mu-RLI蛋白的氨基酸同一性为98%。Mu-RLI在功能上与Hu-RLI同源，Hu-RLI的所有结构特征和氨基酸序列基序在Mu-RLI中均保守。此外，网织红细胞裂解物翻译的Mu-RLI蛋白也能够抑制2-5A与依赖2-5A的RNAse-L的结合。Northern印迹分析显示，Mu-RLI cDNA与一个3.5 kb的mRNA杂交，不过在睾丸中检测到分别为3.5 kb和2.1 kb的两种mRNA，这表明存在组织特异性调控。
