人烟酰胺核苷酸转氢酶的克隆及推导的氨基酸序列

Cloning and deduced amino acid sequence of human nicotinamide nucleotide transhydrogenase.

作者信息

Zieger B, Ware J

机构信息

Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA.

出版信息

DNA Seq. 1997;7(6):369-73. doi: 10.3109/10425179709034058.

DOI:10.3109/10425179709034058

PMID:9524818

Abstract

Pyridine nucleotide transhydrogenases (E.C.1.6.1.1) are integral proteins of the inner mitochondrial membrane. These enzymes are part of the energy-transfer system of the respiratory chain and specifically catalyze the transfer of a hydride ion between nicotinamide adenine dinucleotide, NAD(H), and oxidized nicotinamide dinucleotide phosphate, NADP(H). Here we report the sequence of full-length cDNA clone containing the coding sequence for human mitochondrial nicotinamide nucleotide transhydrogenase. The characterized cDNA contains an insert of 4,232 base pairs with a 91% sequence identity to a previously characterized bovine transhydrogenase cDNA. The deduced human polypeptide sequence displays a high degree of sequence similarity, 97%, to the bovine polypeptide and a comparison of the two sequences is presented.

摘要

吡啶核苷酸转氢酶（E.C.1.6.1.1）是线粒体内膜的整合蛋白。这些酶是呼吸链能量转移系统的一部分，专门催化氢负离子在烟酰胺腺嘌呤二核苷酸（NAD(H)）和氧化型烟酰胺二核苷酸磷酸（NADP(H)）之间的转移。在此，我们报道了包含人类线粒体烟酰胺核苷酸转氢酶编码序列的全长cDNA克隆的序列。所鉴定的cDNA含有一个4232个碱基对的插入片段，与先前鉴定的牛转氢酶cDNA的序列同一性为91%。推导的人类多肽序列与牛多肽显示出高度的序列相似性，为97%，并对这两个序列进行了比较。