产生了一种与猫杯状病毒野毒株广泛反应的单克隆抗体。
Production of a monoclonal antibody reacted broadly with feline calicivirus field isolates.
作者信息
Tajima T, Yoshizaki S, Nakata E, Tohya Y, Ishiguro S, Fujikawa Y, Sugii S
机构信息
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University, Japan.
出版信息
J Vet Med Sci. 1998 Feb;60(2):155-60. doi: 10.1292/jvms.60.155.
A monoclonal antibody (MAb) reactive with 36 field isolates and 2 laboratory strains of feline calicivirus (FCV) was produced by immunizing mice with the mixture of FCVs. The MAb (4D7) reacted with FCVs in an enzyme-linked immunosorbent assay (ELISA), but had no neutralizing activity against the F4 strain of FCV. MAb 8G1, previously produced against the FCV F4 strain, also reacted in ELISA with all FCVs used in the present study. However, the epitopes recognized by 4D7 and 8G1 were different. Using these two MAbs and a polyclonal rabbit antibody, we attempted to develop a sandwich ELISA for detection of FCV antigen. The combination of 4D7 and the polyclonal rabbit IgG was most sensitive. Using this system, all the field isolates of FCV cultured in vitro were detected. However, among the 36 swab samples, from which FCV was isolated, 4 were negative.
用猫杯状病毒（FCV）混合物免疫小鼠，制备了一种与36株野毒株和2株实验室毒株的FCV发生反应的单克隆抗体（MAb）。该单克隆抗体（4D7）在酶联免疫吸附测定（ELISA）中与FCV发生反应，但对FCV F4株没有中和活性。先前针对FCV F4株产生的单克隆抗体8G1在ELISA中也与本研究中使用的所有FCV发生反应。然而，4D7和8G1识别的表位不同。使用这两种单克隆抗体和一种兔多克隆抗体，我们试图开发一种夹心ELISA用于检测FCV抗原。4D7和兔多克隆IgG的组合最为敏感。使用该系统，检测到了所有体外培养的FCV野毒株。然而，在分离出FCV的36份拭子样本中，有4份呈阴性。
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