O'Leary S J, Moore D R
University Laboratory of Physiology, Oxford, England.
Ann Otol Rhinol Laryngol. 1998 Mar;107(3):220-6. doi: 10.1177/000348949810700306.
This study examined the temporal relationship between aminoglycoside ototoxicity and the onset of auditory function in the rat. A single dose of gentamicin sulfate (200 mg/kg) and furosemide (100 mg/kg) was administered on postnatal day 6 (P6), P7, P8, P9, or P10, just before the onset of auditory function. Ototoxicity was assessed by the elevation of auditory brain stem response (ABR) thresholds, recorded once the rats had matured. The ABRs were evoked by acoustic clicks and tone pips. The thresholds of control and P6- and P7-treated animals did not differ significantly from each other. Thresholds of some P8- and all P9-treated animals were elevated. The P10-treated animals were deafened, according to these ABR criteria. These data suggest that the potential for aminoglycoside ototoxicity develops rapidly between P8 and P10, just before the onset of auditory function.
本研究检测了大鼠中氨基糖苷类耳毒性与听觉功能开始之间的时间关系。在出生后第6天（P6）、P7、P8、P9或P10，恰好在听觉功能开始之前，给予单次剂量的硫酸庆大霉素（200 mg/kg）和速尿（100 mg/kg）。耳毒性通过听性脑干反应（ABR）阈值的升高来评估，在大鼠成熟后记录一次。ABR由声点击和声短音诱发。对照组以及P6和P7处理组动物的阈值彼此之间无显著差异。一些P8处理组和所有P9处理组动物的阈值升高。根据这些ABR标准，P10处理组动物失聪。这些数据表明，氨基糖苷类耳毒性的可能性在P8和P10之间迅速发展，恰好在听觉功能开始之前。
