Yagami T, Sato M, Nakamura A, Komiyama T, Kitagawa K, Akasawa A, Ikezawa Z
Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
J Allergy Clin Immunol. 1998 Mar;101(3):379-85. doi: 10.1016/S0091-6749(98)70251-9.
Latex allergy is an increasing hazard to people who frequently come into contact with latex products. Of interest concerning this immediate-type allergy is the cross-reactivity to various vegetable foods and pollen. Despite its high prevalence, no adequate explanation has been provided for the cross-reactive antigens.
We have hypothesized that a series of plant defense-related proteins act as latex allergens, as well as vegetable food allergens. To evaluate this hypothesis, hydrolytic enzymes that are very likely to take on defensive roles in rubber trees were examined for their antigenicity.
By applying chromatographic procedures, defense-related enzymes were separated from nonammoniated latex (NAL). Their antigenicity was examined by immunoblotting and ELISA with sera containing IgE antibodies to crude latex proteins.
Three kinds of hydrolytic enzymes (basic beta-1,3-glucanases [35, 36.5, and 38 kd], a basic chitinase/lysozyme [29.5 kd], and an acidic esterase [44 kd]) were separated from NAL. They were recognized by IgE antibodies from a significant number of patients allergic to latex. The basic beta-1,3-glucanases and the acidic esterase were also strongly recognized by IgE antibodies from several atopic subjects who were allergic to various vegetable foods rather than latex products.
It was ascertained that the three defense-related enzymes separated from NAL constituted part of the latex antigens. Taking together the well-known serologic or immunologic relationships and amino acid sequence similarities of defense-related proteins coming from phylogenetically distant plant species, we can suspect their universal antigenicity and cross-reactivity.
乳胶过敏对经常接触乳胶产品的人来说是一种日益严重的危害。关于这种速发型过敏，令人感兴趣的是其与各种蔬菜食物和花粉的交叉反应性。尽管其患病率很高，但对于交叉反应性抗原尚未给出充分的解释。
我们推测一系列与植物防御相关的蛋白质充当乳胶过敏原以及蔬菜食物过敏原。为了评估这一假设，对很可能在橡胶树中起防御作用的水解酶进行了抗原性检测。
通过色谱程序，从非氨化乳胶（NAL）中分离出与防御相关的酶。用含有针对粗乳胶蛋白的IgE抗体的血清，通过免疫印迹和酶联免疫吸附测定法检测其抗原性。
从NAL中分离出三种水解酶（碱性β-1,3-葡聚糖酶[35、36.5和38千道尔顿]、一种碱性几丁质酶/溶菌酶[29.5千道尔顿]和一种酸性酯酶[44千道尔顿]）。它们被大量对乳胶过敏的患者的IgE抗体识别。碱性β-1,3-葡聚糖酶和酸性酯酶也被几名对各种蔬菜食物而非乳胶产品过敏的特应性受试者的IgE抗体强烈识别。
已确定从NAL中分离出的三种与防御相关的酶构成了乳胶抗原的一部分。综合来自系统发育上遥远植物物种的防御相关蛋白质的众所周知的血清学或免疫学关系以及氨基酸序列相似性，我们可以怀疑它们具有普遍的抗原性和交叉反应性。
